Sandbox Reserved 703: Difference between revisions
No edit summary |
No edit summary |
||
| Line 25: | Line 25: | ||
The <scene name='Sandbox_Reserved_703/Active_center1/1'>active site</scene> is similar to other phosphate SBPs and the surface of the binding pocket is negative. | The <scene name='Sandbox_Reserved_703/Active_center1/1'>active site</scene> is similar to other phosphate SBPs and the surface of the binding pocket is negative. | ||
In total, 8 residues and 13 hydrogen bonds are involved in the binding of the substrate creating a rich network. 12 bonds are formed by dipolar donor groups with <scene name='Sandbox_Reserved_703/6n_backbones/1'>6 backbone NHs</scene> (T8, L9, S32, S144, G145, T146) mostly located at the end of 3 helices, <scene name='Sandbox_Reserved_703/2nh_r140/1'>2 NHs</scene> of the R140 side chain and 4 from side chains OHs of 4 residues(T8, S32, S144, T146). | In total, 8 residues and 13 hydrogen bonds are involved in the binding of the substrate creating a rich network. 12 bonds are formed by dipolar donor groups with <scene name='Sandbox_Reserved_703/6n_backbones/1'>6 backbone NHs</scene> (T8, L9, S32, S144, G145, T146) mostly located at the end of 3 helices, <scene name='Sandbox_Reserved_703/2nh_r140/1'>2 NHs</scene> of the R140 side chain and 4 from side chains OHs of 4 residues (T8, S32, S144, T146). | ||
R140 generates two hydrogen bonds with both negative oxygen atoms of the dibasic phosphate. This interaction, by diminishing the charge coupling interaction between the guanidium and phosphate, has previously been described as essential for the fast release of the ionic substrate bound with charged residues. | R140 generates two hydrogen bonds with both negative oxygen atoms of the dibasic phosphate. This interaction, by diminishing the charge coupling interaction between the guanidium and phosphate, has previously been described as essential for the fast release of the ionic substrate bound with charged residues. | ||