1gv7: Difference between revisions
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Revision as of 16:15, 30 October 2007
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ARH-I, AN ANGIOGENIN/RNASE A CHIMERA
OverviewOverview
Angiogenin and ribonuclease A share 33% sequence identity but have, distinct functions. Angiogenin is a potent inducer of angiogenesis that is, only weakly ribonucleolytic, whereas ribonuclease A is a robust, ribonuclease that is not angiogenic. A chimera ("ARH-I"), in which, angiogenin residues 58-70 are replaced with residues 59-73 of ribonuclease, A, has intermediate ribonucleolytic potency and no angiogenic activity., Here we report a crystal structure of ARH-I that reveals the molecular, basis for these characteristics. The ribonuclease A-derived (guest), segment adopts a structure largely similar to that in ribonuclease A, and, successfully converts this region from a cell-binding site to a, purine-binding site. At the same time, its presence causes complex changes, in the ... [(full description)]
About this StructureAbout this Structure
1GV7 is a [Single protein] structure of sequence from [Homo sapiens, bos taurus] with CIT as [ligand]. Active as [Pancreatic ribonuclease], with EC number [3.1.27.5]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Guest-host crosstalk in an angiogenin-RNase A chimeric protein., Holloway DE, Shapiro R, Hares MC, Leonidas DD, Acharya KR, Biochemistry. 2002 Aug 20;41(33):10482-9. PMID:12173935
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