1v9y: Difference between revisions

Revision as of 16:33, 21 February 2008

Crystal Structure of the heme PAS sensor domain of Ec DOS (ferric form)

OverviewOverview

PAS domains, which have been identified in over 1100 proteins from all three kingdoms of life, convert various input stimuli into signals that propagate to downstream components by modifying protein-protein interactions. One such protein is the Escherichia coli redox sensor, Ec DOS, a phosphodiesterase that degrades cyclic adenosine monophosphate in a redox-dependent manner. Here we report the crystal structures of the heme PAS domain of Ec DOS in both inactive Fe(3+) and active Fe(2+) forms at 1.32 and 1.9 A resolution, respectively. The protein folds into a characteristic PAS domain structure and forms a homodimer. In the Fe(3+) form, the heme iron is ligated to a His-77 side chain and a water molecule. Heme iron reduction is accompanied by heme-ligand switching from the water molecule to a side chain of Met-95 from the FG loop. Concomitantly, the flexible FG loop is significantly rigidified, along with a change in the hydrogen bonding pattern and rotation of subunits relative to each other. The present data led us to propose a novel redox-regulated molecular switch in which local heme-ligand switching may trigger a global "scissor-type" subunit movement that facilitates catalytic control.

About this StructureAbout this Structure

1V9Y is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

A redox-controlled molecular switch revealed by the crystal structure of a bacterial heme PAS sensor., Kurokawa H, Lee DS, Watanabe M, Sagami I, Mikami B, Raman CS, Shimizu T, J Biol Chem. 2004 May 7;279(19):20186-93. Epub 2004 Feb 23. PMID:14982921

Page seeded by OCA on Thu Feb 21 15:33:03 2008

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OCA

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-[[Image:1v9y.jpg|left|200px]]<br /><applet load="1v9y" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1v9y.jpg|left|200px]]<br /><applet load="1v9y" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1v9y, resolution 1.32&Aring;" />
 '''Crystal Structure of the heme PAS sensor domain of Ec DOS (ferric form)'''<br />
 ==Overview==
-PAS domains, which have been identified in over 1100 proteins from all, three kingdoms of life, convert various input stimuli into signals that, propagate to downstream components by modifying protein-protein, interactions. One such protein is the Escherichia coli redox sensor, Ec, DOS, a phosphodiesterase that degrades cyclic adenosine monophosphate in a, redox-dependent manner. Here we report the crystal structures of the heme, PAS domain of Ec DOS in both inactive Fe(3+) and active Fe(2+) forms at, 1.32 and 1.9 A resolution, respectively. The protein folds into a, characteristic PAS domain structure and forms a homodimer. In the Fe(3+), form, the heme iron is ligated to a His-77 side chain and a water, molecule. Heme iron reduction is accompanied by heme-ligand switching from, the water molecule to a side chain of Met-95 from the FG loop., Concomitantly, the flexible FG loop is significantly rigidified, along, with a change in the hydrogen bonding pattern and rotation of subunits, relative to each other. The present data led us to propose a novel, redox-regulated molecular switch in which local heme-ligand switching may, trigger a global "scissor-type" subunit movement that facilitates, catalytic control.
+PAS domains, which have been identified in over 1100 proteins from all three kingdoms of life, convert various input stimuli into signals that propagate to downstream components by modifying protein-protein interactions. One such protein is the Escherichia coli redox sensor, Ec DOS, a phosphodiesterase that degrades cyclic adenosine monophosphate in a redox-dependent manner. Here we report the crystal structures of the heme PAS domain of Ec DOS in both inactive Fe(3+) and active Fe(2+) forms at 1.32 and 1.9 A resolution, respectively. The protein folds into a characteristic PAS domain structure and forms a homodimer. In the Fe(3+) form, the heme iron is ligated to a His-77 side chain and a water molecule. Heme iron reduction is accompanied by heme-ligand switching from the water molecule to a side chain of Met-95 from the FG loop. Concomitantly, the flexible FG loop is significantly rigidified, along with a change in the hydrogen bonding pattern and rotation of subunits relative to each other. The present data led us to propose a novel redox-regulated molecular switch in which local heme-ligand switching may trigger a global "scissor-type" subunit movement that facilitates catalytic control.
 ==About this Structure==
-V9Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V9Y OCA].
+V9Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9Y OCA].
 ==Reference==
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 [[Category: Single protein]]
 [[Category: Kurokawa, H.]]
-[[Category: Lee, D.S.]]
+[[Category: Lee, D S.]]
 [[Category: Mikami, B.]]
-[[Category: Raman, C.S.]]
+[[Category: Raman, C S.]]
 [[Category: Sagami, I.]]
 [[Category: Shimizu, T.]]
@@ Line 25: / Line 25: @@
 [[Category: sensor]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:32:19 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:33:03 2008''