1uw7: Difference between revisions

Revision as of 16:29, 21 February 2008

NSP9 PROTEIN FROM SARS-CORONAVIRUS.

OverviewOverview

As part of a high-throughput structural analysis of SARS-coronavirus (SARS-CoV) proteins, we have solved the structure of the non-structural protein 9 (nsp9). This protein, encoded by ORF1a, has no designated function but is most likely involved with viral RNA synthesis. The protein comprises a single beta-barrel with a fold previously unseen in single domain proteins. The fold superficially resembles an OB-fold with a C-terminal extension and is related to both of the two subdomains of the SARS-CoV 3C-like protease (which belongs to the serine protease superfamily). nsp9 has, presumably, evolved from a protease. The crystal structure suggests that the protein is dimeric. This is confirmed by analytical ultracentrifugation and dynamic light scattering. We show that nsp9 binds RNA and interacts with nsp8, activities that may be essential for its function(s).

About this StructureAbout this Structure

1UW7 is a Single protein structure of sequence from Sars coronavirus hku-39849. Full crystallographic information is available from OCA.

ReferenceReference

The nsp9 replicase protein of SARS-coronavirus, structure and functional insights., Sutton G, Fry E, Carter L, Sainsbury S, Walter T, Nettleship J, Berrow N, Owens R, Gilbert R, Davidson A, Siddell S, Poon LL, Diprose J, Alderton D, Walsh M, Grimes JM, Stuart DI, Structure. 2004 Feb;12(2):341-53. PMID:14962394

Page seeded by OCA on Thu Feb 21 15:29:02 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1uw7.gif|left|200px]]<br /><applet load="1uw7" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1uw7.gif|left|200px]]<br /><applet load="1uw7" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1uw7, resolution 2.8&Aring;" />
 '''NSP9 PROTEIN FROM SARS-CORONAVIRUS.'''<br />
 ==Overview==
-As part of a high-throughput structural analysis of SARS-coronavirus, (SARS-CoV) proteins, we have solved the structure of the non-structural, protein 9 (nsp9). This protein, encoded by ORF1a, has no designated, function but is most likely involved with viral RNA synthesis. The protein, comprises a single beta-barrel with a fold previously unseen in single, domain proteins. The fold superficially resembles an OB-fold with a, C-terminal extension and is related to both of the two subdomains of the, SARS-CoV 3C-like protease (which belongs to the serine protease, superfamily). nsp9 has, presumably, evolved from a protease. The crystal, structure suggests that the protein is dimeric. This is confirmed by, analytical ultracentrifugation and dynamic light scattering. We show that, nsp9 binds RNA and interacts with nsp8, activities that may be essential, for its function(s).
+As part of a high-throughput structural analysis of SARS-coronavirus (SARS-CoV) proteins, we have solved the structure of the non-structural protein 9 (nsp9). This protein, encoded by ORF1a, has no designated function but is most likely involved with viral RNA synthesis. The protein comprises a single beta-barrel with a fold previously unseen in single domain proteins. The fold superficially resembles an OB-fold with a C-terminal extension and is related to both of the two subdomains of the SARS-CoV 3C-like protease (which belongs to the serine protease superfamily). nsp9 has, presumably, evolved from a protease. The crystal structure suggests that the protein is dimeric. This is confirmed by analytical ultracentrifugation and dynamic light scattering. We show that nsp9 binds RNA and interacts with nsp8, activities that may be essential for its function(s).
 ==About this Structure==
-UW7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus_hku-39849 Sars coronavirus hku-39849]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UW7 OCA].
+UW7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus_hku-39849 Sars coronavirus hku-39849]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UW7 OCA].
 ==Reference==
@@ Line 20: / Line 20: @@
 [[Category: Fry, E.]]
 [[Category: Gilbert, R.]]
-[[Category: Grimes, J.M.]]
+[[Category: Grimes, J M.]]
 [[Category: Nettleship, J.]]
 [[Category: Owens, R.]]
-[[Category: Poon, L.L.M.]]
+[[Category: Poon, L L.M.]]
 [[Category: Sainsbury, S.]]
 [[Category: Siddell, S.]]
-[[Category: Stuart, D.I.]]
+[[Category: Stuart, D I.]]
 [[Category: Sutton, G.]]
 [[Category: Walsh, M.]]
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 [[Category: virus]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:20:44 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:29:02 2008''