Sandbox Reserved 711: Difference between revisions
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Asp180 and Asp202 bind the water molecule used in the hydrolyse and activate it, while Asp201, which is less exposed to the aqueous solvent, is stabilized its protonated state. In theses conditions, a bond can be built between the oxygen of the water and the carbon 1 of the galacturonic acid, whereas the bond between this carbon and the oxygen of the glycosidic bond is cleaved, the oxygen taking the proton released by Asp201.<ref>http://www.jbc.org/content/275/38/29348.short</ref> | Asp180 and Asp202 bind the water molecule used in the hydrolyse and activate it, while Asp201, which is less exposed to the aqueous solvent, is stabilized its protonated state. In theses conditions, a bond can be built between the oxygen of the water and the carbon 1 of the galacturonic acid, whereas the bond between this carbon and the oxygen of the glycosidic bond is cleaved, the oxygen taking the proton released by Asp201.<ref>http://www.jbc.org/content/275/38/29348.short</ref> | ||
[[Image:proposed mechanism.png | | [[Image:proposed mechanism.png | 332 px |center|thumb|'''proposed mechanism for family 28 of glycoside hydrolases''']] | ||
== Description of the structure == | == Description of the structure == | ||