1ulf: Difference between revisions

Revision as of 16:25, 21 February 2008

CGL2 in complex with Blood Group A tetrasaccharide

OverviewOverview

Recognition of and discrimination between potential glyco-substrates is central to the function of galectins. Here we dissect the fundamental parameters responsible for such selectivity by the fungal representative, CGL2. The 2.1 A crystal structure of CGL2 and five substrate complexes reveal that this prototype galectin achieves increased substrate specificity by accommodating substituted oligosaccharides of the mammalian blood group A/B type in an extended binding cleft. Kinetic studies on wild-type and mutant CGL2 proteins demonstrate that the tetrameric organization is essential for functionality. The geometric constraints due to the orthogonal orientation of the four binding sites have important consequences on substrate binding and selectivity.

About this StructureAbout this Structure

1ULF is a Single protein structure of sequence from Coprinopsis cinerea. Full crystallographic information is available from OCA.

ReferenceReference

Structure and functional analysis of the fungal galectin CGL2., Walser PJ, Haebel PW, Kunzler M, Sargent D, Kues U, Aebi M, Ban N, Structure. 2004 Apr;12(4):689-702. PMID:15062091

Page seeded by OCA on Thu Feb 21 15:25:45 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 '''CGL2 in complex with Blood Group A tetrasaccharide'''<br />
 ==Overview==
-Recognition of and discrimination between potential glyco-substrates is, central to the function of galectins. Here we dissect the fundamental, parameters responsible for such selectivity by the fungal representative, CGL2. The 2.1 A crystal structure of CGL2 and five substrate complexes, reveal that this prototype galectin achieves increased substrate, specificity by accommodating substituted oligosaccharides of the mammalian, blood group A/B type in an extended binding cleft. Kinetic studies on, wild-type and mutant CGL2 proteins demonstrate that the tetrameric, organization is essential for functionality. The geometric constraints due, to the orthogonal orientation of the four binding sites have important, consequences on substrate binding and selectivity.
+Recognition of and discrimination between potential glyco-substrates is central to the function of galectins. Here we dissect the fundamental parameters responsible for such selectivity by the fungal representative, CGL2. The 2.1 A crystal structure of CGL2 and five substrate complexes reveal that this prototype galectin achieves increased substrate specificity by accommodating substituted oligosaccharides of the mammalian blood group A/B type in an extended binding cleft. Kinetic studies on wild-type and mutant CGL2 proteins demonstrate that the tetrameric organization is essential for functionality. The geometric constraints due to the orthogonal orientation of the four binding sites have important consequences on substrate binding and selectivity.
 ==About this Structure==
-ULF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ULF OCA].
+ULF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ULF OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Aebi, M.]]
 [[Category: Ban, N.]]
-[[Category: Haebel, P.W.]]
+[[Category: Haebel, P W.]]
 [[Category: Kuenzler, M.]]
 [[Category: Kues, U.]]
-[[Category: Walser, P.J.]]
+[[Category: Walser, P J.]]
 [[Category: beta-galactoside binding lectin]]
 [[Category: galectin]]
@@ Line 24: / Line 24: @@
 [[Category: sugar binding]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:13:26 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:25:45 2008''