1udv: Difference between revisions

Revision as of 16:23, 21 February 2008

Crystal structure of the hyperthermophilic archaeal dna-binding protein Sso10b2 at 1.85 A

OverviewOverview

The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure is similar to that of Sso10b1 (also called Alba) from the same organism. However, Sso10b2 contains an arginine-rich loop RDRRR motif, which may play an important role in nucleic acid binding. There are two independent Sso10b2 proteins in the asymmetric unit, and a plausible stable dimer could be deduced from the crystal structure. Topology comparison revealed that Sso10b2 is similar to several RNA-binding proteins, including IF3-C, YhhP, and DNase I. Models of the Sso10b2 dimer bound to either B-DNA or A-DNA have been constructed.

About this StructureAbout this Structure

1UDV is a Single protein structure of sequence from Sulfolobus solfataricus with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the hyperthermophilic archaeal DNA-binding protein Sso10b2 at a resolution of 1.85 Angstroms., Chou CC, Lin TW, Chen CY, Wang AH, J Bacteriol. 2003 Jul;185(14):4066-73. PMID:12837780

Page seeded by OCA on Thu Feb 21 15:23:26 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1udv.jpg|left|200px]]<br /><applet load="1udv" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1udv.jpg|left|200px]]<br /><applet load="1udv" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1udv, resolution 1.85&Aring;" />
 '''Crystal structure of the hyperthermophilic archaeal dna-binding protein Sso10b2 at 1.85 A'''<br />
 ==Overview==
-The crystal structure of a small, basic DNA binding protein, Sso10b2, from, the thermoacidophilic archaeon Sulfolobus solfataricus was determined by, the Zn multiwavelength anomalous diffraction method and refined to 1.85 A, resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta, topology. The structure is similar to that of Sso10b1 (also called Alba), from the same organism. However, Sso10b2 contains an arginine-rich loop, RDRRR motif, which may play an important role in nucleic acid binding., There are two independent Sso10b2 proteins in the asymmetric unit, and a, plausible stable dimer could be deduced from the crystal structure., Topology comparison revealed that Sso10b2 is similar to several, RNA-binding proteins, including IF3-C, YhhP, and DNase I. Models of the, Sso10b2 dimer bound to either B-DNA or A-DNA have been constructed.
+The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure is similar to that of Sso10b1 (also called Alba) from the same organism. However, Sso10b2 contains an arginine-rich loop RDRRR motif, which may play an important role in nucleic acid binding. There are two independent Sso10b2 proteins in the asymmetric unit, and a plausible stable dimer could be deduced from the crystal structure. Topology comparison revealed that Sso10b2 is similar to several RNA-binding proteins, including IF3-C, YhhP, and DNase I. Models of the Sso10b2 dimer bound to either B-DNA or A-DNA have been constructed.
 ==About this Structure==
-UDV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UDV OCA].
+UDV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UDV OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Sulfolobus solfataricus]]
-[[Category: Chen, C.Y.]]
+[[Category: Chen, C Y.]]
-[[Category: Chou, C.C.]]
+[[Category: Chou, C C.]]
-[[Category: Lin, T.W.]]
+[[Category: Lin, T W.]]
-[[Category: Wang, A.H.J.]]
+[[Category: Wang, A H.J.]]
 [[Category: ZN]]
 [[Category: dna binding protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:03:14 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:23:26 2008''