1u0r: Difference between revisions
New page: left|200px<br /><applet load="1u0r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u0r, resolution 2.80Å" /> '''Crystal structure of... |
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[[Image:1u0r.gif|left|200px]]<br /><applet load="1u0r" size=" | [[Image:1u0r.gif|left|200px]]<br /><applet load="1u0r" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1u0r, resolution 2.80Å" /> | caption="1u0r, resolution 2.80Å" /> | ||
'''Crystal structure of Mycobacterium tuberculosis NAD kinase'''<br /> | '''Crystal structure of Mycobacterium tuberculosis NAD kinase'''<br /> | ||
==Overview== | ==Overview== | ||
NAD kinase catalyzes the magnesium-dependent phosphorylation of NAD, representing the sole source of freshly synthesized NADP in all organisms. | NAD kinase catalyzes the magnesium-dependent phosphorylation of NAD, representing the sole source of freshly synthesized NADP in all organisms. The enzyme is essential for the growth of the deadly multidrug-resistant pathogen Mycobacterium tuberculosis and is an attractive target for novel antitubercular agents. The crystal structure of NAD kinase has been solved by multiwavelength anomalous dispersion at a resolution of 2.3 A in its T state. Two crystal forms have been obtained revealing either a dimer or a tetramer. The enzyme architecture discloses a novel molecular arrangement, with each subunit consisting of an alpha/beta N-terminal domain and a C-terminal 12-stranded beta sandwich domain, connected by swapped beta strands. The C-terminal domain shows a striking internal approximate 222 symmetry and an unprecedented topology, revealing a novel fold within the family of all beta structures. The catalytic site is located in the long crevice that defines the interface between the domains. The conserved GGDG structural fingerprint of the catalytic site is reminiscent of the related region in 6-phosphofructokinase, supporting the hypothesis that NAD kinase belongs to a newly reported superfamily of kinases. | ||
==About this Structure== | ==About this Structure== | ||
1U0R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Active as [http://en.wikipedia.org/wiki/NAD(+)_kinase NAD(+) kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.23 2.7.1.23] Full crystallographic information is available from [http:// | 1U0R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Active as [http://en.wikipedia.org/wiki/NAD(+)_kinase NAD(+) kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.23 2.7.1.23] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0R OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Raffaelli, N.]] | [[Category: Raffaelli, N.]] | ||
[[Category: Rizzi, M.]] | [[Category: Rizzi, M.]] | ||
[[Category: TBSGC, TB | [[Category: TBSGC, TB Structural Genomics Consortium.]] | ||
[[Category: alpha-beta; beta sandwich]] | [[Category: alpha-beta; beta sandwich]] | ||
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
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[[Category: tbsgc]] | [[Category: tbsgc]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:19:25 2008'' | ||
Revision as of 16:19, 21 February 2008
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Crystal structure of Mycobacterium tuberculosis NAD kinase
OverviewOverview
NAD kinase catalyzes the magnesium-dependent phosphorylation of NAD, representing the sole source of freshly synthesized NADP in all organisms. The enzyme is essential for the growth of the deadly multidrug-resistant pathogen Mycobacterium tuberculosis and is an attractive target for novel antitubercular agents. The crystal structure of NAD kinase has been solved by multiwavelength anomalous dispersion at a resolution of 2.3 A in its T state. Two crystal forms have been obtained revealing either a dimer or a tetramer. The enzyme architecture discloses a novel molecular arrangement, with each subunit consisting of an alpha/beta N-terminal domain and a C-terminal 12-stranded beta sandwich domain, connected by swapped beta strands. The C-terminal domain shows a striking internal approximate 222 symmetry and an unprecedented topology, revealing a novel fold within the family of all beta structures. The catalytic site is located in the long crevice that defines the interface between the domains. The conserved GGDG structural fingerprint of the catalytic site is reminiscent of the related region in 6-phosphofructokinase, supporting the hypothesis that NAD kinase belongs to a newly reported superfamily of kinases.
About this StructureAbout this Structure
1U0R is a Single protein structure of sequence from Mycobacterium tuberculosis. Active as NAD(+) kinase, with EC number 2.7.1.23 Full crystallographic information is available from OCA.
ReferenceReference
A novel fold revealed by Mycobacterium tuberculosis NAD kinase, a key allosteric enzyme in NADP biosynthesis., Garavaglia S, Raffaelli N, Finaurini L, Magni G, Rizzi M, J Biol Chem. 2004 Sep 24;279(39):40980-6. Epub 2004 Jul 21. PMID:15269221
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