1twc: Difference between revisions

Revision as of 16:18, 21 February 2008

RNA polymerase II complexed with GTP

OverviewOverview

Binding of a ribonucleoside triphosphate to an RNA polymerase II transcribing complex, with base pairing to the template DNA, was revealed by X-ray crystallography. Binding of a mismatched nucleoside triphosphate was also detected, but in an adjacent site, inverted with respect to the correctly paired nucleotide. The results are consistent with a two-step mechanism of nucleotide selection, with initial binding to an entry (E) site beneath the active center in an inverted orientation, followed by rotation into the nucleotide addition (A) site for pairing with the template DNA. This mechanism is unrelated to that of single subunit RNA polymerases and so defines a new paradigm for the large, multisubunit enzymes. Additional findings from these studies include a third nucleotide binding site that may define the length of backtracked RNA; DNA double helix unwinding in advance of the polymerase active center; and extension of the diffraction limit of RNA polymerase II crystals to 2.3 A.

About this StructureAbout this Structure

1TWC is a Protein complex structure of sequences from Saccharomyces cerevisiae with , and as ligands. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of transcription: nucleotide selection by rotation in the RNA polymerase II active center., Westover KD, Bushnell DA, Kornberg RD, Cell. 2004 Nov 12;119(4):481-9. PMID:15537538

Page seeded by OCA on Thu Feb 21 15:18:02 2008

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OCA

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-[[Image:1twc.gif|left|200px]]<br /><applet load="1twc" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1twc.gif|left|200px]]<br /><applet load="1twc" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1twc, resolution 3.0&Aring;" />
 '''RNA polymerase II complexed with GTP'''<br />
 ==Overview==
-Binding of a ribonucleoside triphosphate to an RNA polymerase II, transcribing complex, with base pairing to the template DNA, was revealed, by X-ray crystallography. Binding of a mismatched nucleoside triphosphate, was also detected, but in an adjacent site, inverted with respect to the, correctly paired nucleotide. The results are consistent with a two-step, mechanism of nucleotide selection, with initial binding to an entry (E), site beneath the active center in an inverted orientation, followed by, rotation into the nucleotide addition (A) site for pairing with the, template DNA. This mechanism is unrelated to that of single subunit RNA, polymerases and so defines a new paradigm for the large, multisubunit, enzymes. Additional findings from these studies include a third nucleotide, binding site that may define the length of backtracked RNA; DNA double, helix unwinding in advance of the polymerase active center; and extension, of the diffraction limit of RNA polymerase II crystals to 2.3 A.
+Binding of a ribonucleoside triphosphate to an RNA polymerase II transcribing complex, with base pairing to the template DNA, was revealed by X-ray crystallography. Binding of a mismatched nucleoside triphosphate was also detected, but in an adjacent site, inverted with respect to the correctly paired nucleotide. The results are consistent with a two-step mechanism of nucleotide selection, with initial binding to an entry (E) site beneath the active center in an inverted orientation, followed by rotation into the nucleotide addition (A) site for pairing with the template DNA. This mechanism is unrelated to that of single subunit RNA polymerases and so defines a new paradigm for the large, multisubunit enzymes. Additional findings from these studies include a third nucleotide binding site that may define the length of backtracked RNA; DNA double helix unwinding in advance of the polymerase active center; and extension of the diffraction limit of RNA polymerase II crystals to 2.3 A.
 ==About this Structure==
-TWC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN, MN and GTP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TWC OCA].
+TWC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=GTP:'>GTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWC OCA].
 ==Reference==
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 [[Category: Protein complex]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Bushnell, D.A.]]
+[[Category: Bushnell, D A.]]
-[[Category: Kornberg, R.D.]]
+[[Category: Kornberg, R D.]]
-[[Category: Westover, K.D.]]
+[[Category: Westover, K D.]]
 [[Category: GTP]]
 [[Category: MN]]
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 [[Category: zinc motifs]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:38:38 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:18:02 2008''