1ttu: Difference between revisions
New page: left|200px<br /><applet load="1ttu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ttu, resolution 2.85Å" /> '''Crystal Structure of... |
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[[Image:1ttu.gif|left|200px]]<br /><applet load="1ttu" size=" | [[Image:1ttu.gif|left|200px]]<br /><applet load="1ttu" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1ttu, resolution 2.85Å" /> | caption="1ttu, resolution 2.85Å" /> | ||
'''Crystal Structure of CSL bound to DNA'''<br /> | '''Crystal Structure of CSL bound to DNA'''<br /> | ||
==Overview== | ==Overview== | ||
Notch signaling is a conserved pathway of communication between | Notch signaling is a conserved pathway of communication between neighboring cells that results in cell fate specification, and CSL is the universal transcriptional effector of Notch signaling. The Notch intracellular domain translocates to the nucleus after proteolytic release upon Notch extracellular engagement, and there it displaces corepressors from DNA-bound CSL and recruits activators of Notch target genes. Here we report the 2.85 A crystal structure of CSL with a target DNA. CSL comprises three structurally integrated domains: its amino (NTD)- and carboxy (CTD)-terminal domains are strikingly similar to those of Rel transcription factors, but a surprising beta-trefoil domain (BTD) is inserted between them. CSL-bound DNA is recognized specifically by conserved residues from NTD and BTD. A hydrophobic pocket on BTD is identified as the likely site of Notch interaction with CSL, which has functional implications for the mechanism of Notch signaling. | ||
==About this Structure== | ==About this Structure== | ||
1TTU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] with EDO as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1TTU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] with <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TTU OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Caenorhabditis elegans]] | [[Category: Caenorhabditis elegans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Hendrickson, W | [[Category: Hendrickson, W A.]] | ||
[[Category: Kovall, R | [[Category: Kovall, R A.]] | ||
[[Category: EDO]] | [[Category: EDO]] | ||
[[Category: beta-trefoil domain]] | [[Category: beta-trefoil domain]] | ||
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[[Category: transcription factor]] | [[Category: transcription factor]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:17:15 2008'' | ||
Revision as of 16:17, 21 February 2008
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Crystal Structure of CSL bound to DNA
OverviewOverview
Notch signaling is a conserved pathway of communication between neighboring cells that results in cell fate specification, and CSL is the universal transcriptional effector of Notch signaling. The Notch intracellular domain translocates to the nucleus after proteolytic release upon Notch extracellular engagement, and there it displaces corepressors from DNA-bound CSL and recruits activators of Notch target genes. Here we report the 2.85 A crystal structure of CSL with a target DNA. CSL comprises three structurally integrated domains: its amino (NTD)- and carboxy (CTD)-terminal domains are strikingly similar to those of Rel transcription factors, but a surprising beta-trefoil domain (BTD) is inserted between them. CSL-bound DNA is recognized specifically by conserved residues from NTD and BTD. A hydrophobic pocket on BTD is identified as the likely site of Notch interaction with CSL, which has functional implications for the mechanism of Notch signaling.
About this StructureAbout this Structure
1TTU is a Single protein structure of sequence from Caenorhabditis elegans with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the nuclear effector of Notch signaling, CSL, bound to DNA., Kovall RA, Hendrickson WA, EMBO J. 2004 Sep 1;23(17):3441-51. Epub 2004 Aug 5. PMID:15297877
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