Sandbox Reserved 714: Difference between revisions

The N-terminal domain is responsible of the Mg²⁺ dependant hydrolysis of p-nitrophenyl phosphate <ref>PMID:15096040</ref>. Its <scene name='Sandbox_Reserved_714/Nter_activesite/1'>active site</scene> contains several conserved aspartates in phosphatases and phosphonatases: D9, D11, D184 and D185. This enzymatic activity is Mg²⁺ dependant, because the structure of the active site is in its optimal conformation when the cation makes coordination interactions. When the catalytic activity of the N-term domain is available, Magnesium is octahedrally coordinated with the four aspartates, one water molecule and the phosphate belonging to the substrate.