1tet: Difference between revisions

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CRYSTAL STRUCTURE OF AN ANTICHOLERA TOXIN PEPTIDE COMPLEX AT 2.3 ANGSTROMS

OverviewOverview

Cholera toxin peptide 3 (CTP3) is a 15-residue peptide corresponding in sequence to an immunogenic loop on the surface of the B-subunits of both cholera toxin and the heat-labile toxin from Escherichia coli. TE33 is the Fab fragment of a monoclonal antibody elicited against CTP3. The crystal structure of the TE33-CTP3 complex at 2.3 A resolution reveals an antigen-binding pocket, 13 A deep and 13 A wide, which is lined with many aromatic residues. The N-terminal portion of the peptide antigen CTP3 forms a type II beta-turn that fits snugly into this pocket. At gln7 the peptide backbone of CTP3 forms a kink followed by an extended C-terminal chain that seals off the cleft and buries the beta-turn underneath it. All six complementarity-determining regions of TE33 contribute to the binding of CTP3. The antibody-peptide contacts include, in addition to van der Waals' interactions and hydrogen bonds, also one salt bridge and one water molecule, which mediates the interaction.

About this StructureAbout this Structure

1TET is a Protein complex structure of sequences from Mus musculus with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of an anticholera toxin peptide complex at 2.3 A., Shoham M, J Mol Biol. 1993 Aug 20;232(4):1169-75. PMID:7690406

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 caption="1tet, resolution 2.3&Aring;" />
 '''CRYSTAL STRUCTURE OF AN ANTICHOLERA TOXIN PEPTIDE COMPLEX AT 2.3 ANGSTROMS'''<br />
 ==Overview==
-Cholera toxin peptide 3 (CTP3) is a 15-residue peptide corresponding in, sequence to an immunogenic loop on the surface of the B-subunits of both, cholera toxin and the heat-labile toxin from Escherichia coli. TE33 is the, Fab fragment of a monoclonal antibody elicited against CTP3. The crystal, structure of the TE33-CTP3 complex at 2.3 A resolution reveals an, antigen-binding pocket, 13 A deep and 13 A wide, which is lined with many, aromatic residues. The N-terminal portion of the peptide antigen CTP3, forms a type II beta-turn that fits snugly into this pocket. At gln7 the, peptide backbone of CTP3 forms a kink followed by an extended C-terminal, chain that seals off the cleft and buries the beta-turn underneath it. All, six complementarity-determining regions of TE33 contribute to the binding, of CTP3. The antibody-peptide contacts include, in addition to van der, Waals' interactions and hydrogen bonds, also one salt bridge and one water, molecule, which mediates the interaction.
+Cholera toxin peptide 3 (CTP3) is a 15-residue peptide corresponding in sequence to an immunogenic loop on the surface of the B-subunits of both cholera toxin and the heat-labile toxin from Escherichia coli. TE33 is the Fab fragment of a monoclonal antibody elicited against CTP3. The crystal structure of the TE33-CTP3 complex at 2.3 A resolution reveals an antigen-binding pocket, 13 A deep and 13 A wide, which is lined with many aromatic residues. The N-terminal portion of the peptide antigen CTP3 forms a type II beta-turn that fits snugly into this pocket. At gln7 the peptide backbone of CTP3 forms a kink followed by an extended C-terminal chain that seals off the cleft and buries the beta-turn underneath it. All six complementarity-determining regions of TE33 contribute to the binding of CTP3. The antibody-peptide contacts include, in addition to van der Waals' interactions and hydrogen bonds, also one salt bridge and one water molecule, which mediates the interaction.
 ==About this Structure==
-TET is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with CIT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TET OCA].
+TET is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TET OCA].
 ==Reference==
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 [[Category: immunoglobulin]]
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