Sandbox Reserved 712: Difference between revisions
| Line 63: | Line 63: | ||
Samples PR<sub>DRV1</sub> to PR<sub>DRV6</sub> have been cloned and expressed in ''E. coli'', purified and characterized in vitro by monitoring cleavage of a chromogenic peptide substrate in the presence and absence of specific PIs. | Samples PR<sub>DRV1</sub> to PR<sub>DRV6</sub> have been cloned and expressed in ''E. coli'', purified and characterized in vitro by monitoring cleavage of a chromogenic peptide substrate in the presence and absence of specific PIs. | ||
PR<sub>DRV5</sub> (3ggu) shows twenty mutated amino acids. The PR mutations that are associated to the darunavir resistance ([[HIV Protease Resistance]]) are <scene name='Sandbox_Reserved_712/L33f/1'>L33F</scene>, <scene name='Sandbox_Reserved_712/I84v/2'>I84V</scene> and | PR<sub>DRV5</sub> (<scene name='Sandbox_Reserved_712/3ggu/1'>3ggu</scene>) shows twenty mutated amino acids. The PR mutations that are associated to the darunavir resistance ([[HIV Protease Resistance]]) are <scene name='Sandbox_Reserved_712/L33f/1'>L33F</scene>, <scene name='Sandbox_Reserved_712/I84v/2'>I84V</scene> and | ||
<scene name='Sandbox_Reserved_712/L89v/1'>L89V</scene>. | <scene name='Sandbox_Reserved_712/L89v/1'>L89V</scene>. | ||