1t1b: Difference between revisions
New page: left|200px<br /><applet load="1t1b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t1b, resolution 1.60Å" /> '''Late intermediate IL... |
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[[Image:1t1b.gif|left|200px]]<br /><applet load="1t1b" size=" | [[Image:1t1b.gif|left|200px]]<br /><applet load="1t1b" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1t1b, resolution 1.60Å" /> | caption="1t1b, resolution 1.60Å" /> | ||
'''Late intermediate IL2 from time-resolved crystallography of the E46Q mutant of PYP'''<br /> | '''Late intermediate IL2 from time-resolved crystallography of the E46Q mutant of PYP'''<br /> | ||
==Overview== | ==Overview== | ||
In the bacterial photoreceptor photoactive yellow protein (PYP), absorption of blue light by its chromophore leads to a conformational | In the bacterial photoreceptor photoactive yellow protein (PYP), absorption of blue light by its chromophore leads to a conformational change in the protein associated with differential signaling activity, as it executes a reversible photocycle. Time-resolved Laue crystallography allows structural snapshots (as short as 150 ps) of high crystallographic resolution (approximately 1.6 A) to be taken of a protein as it functions. Here, we analyze by singular value decomposition a comprehensive time-resolved crystallographic data set of the E46Q mutant of PYP throughout the photocycle spanning 10 ns-100 ms. We identify and refine the structures of five distinct intermediates and provide a plausible chemical kinetic mechanism for their inter conversion. A clear structural progression is visible in these intermediates, in which a signal generated at the chromophore propagates through a distinct structural pathway of conserved residues and results in structural changes near the N terminus, over 20 A distant from the chromophore. | ||
==About this Structure== | ==About this Structure== | ||
1T1B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila] with HC4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1T1B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila] with <scene name='pdbligand=HC4:'>HC4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1B OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: photoactive yellow protein]] | [[Category: photoactive yellow protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:08:50 2008'' | ||
Revision as of 16:08, 21 February 2008
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Late intermediate IL2 from time-resolved crystallography of the E46Q mutant of PYP
OverviewOverview
In the bacterial photoreceptor photoactive yellow protein (PYP), absorption of blue light by its chromophore leads to a conformational change in the protein associated with differential signaling activity, as it executes a reversible photocycle. Time-resolved Laue crystallography allows structural snapshots (as short as 150 ps) of high crystallographic resolution (approximately 1.6 A) to be taken of a protein as it functions. Here, we analyze by singular value decomposition a comprehensive time-resolved crystallographic data set of the E46Q mutant of PYP throughout the photocycle spanning 10 ns-100 ms. We identify and refine the structures of five distinct intermediates and provide a plausible chemical kinetic mechanism for their inter conversion. A clear structural progression is visible in these intermediates, in which a signal generated at the chromophore propagates through a distinct structural pathway of conserved residues and results in structural changes near the N terminus, over 20 A distant from the chromophore.
About this StructureAbout this Structure
1T1B is a Single protein structure of sequence from Halorhodospira halophila with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
A structural pathway for signaling in the E46Q mutant of photoactive yellow protein., Rajagopal S, Anderson S, Srajer V, Schmidt M, Pahl R, Moffat K, Structure. 2005 Jan;13(1):55-63. PMID:15642261
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