1e9m: Difference between revisions
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[[Category: ferredoxin]] | [[Category: ferredoxin]] | ||
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Revision as of 16:04, 30 October 2007
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FERREDOXIN VI FROM RHODOBACTER CAPSULATUS
OverviewOverview
A [2Fe-2S] ferredoxin found in the photosynthetic bacterium Rhodobacter, capsulatus has been purified in recombinant form from Escherichia coli., This protein, called FdVI, resembles ferredoxins involved in iron-sulfur, cluster biosynthesis in various prokaryotic and eukaryotic cells. Purified, recombinant FdVI was recovered in high yields and appeared to be, indistinguishable from the genuine R. capsulatus ferredoxin based on, UV-visible absorption and EPR spectroscopy and mass spectrometry. FdVI has, been crystallized in the oxidized state by a sitting-drop vapour-diffusion, technique using sodium formate as precipitant. Seeding larger drops from a, previous hanging-drop-grown small crystal resulted in the formation of, long red-brown prismatic needles. Preliminary X-ray diffraction ... [(full description)]
About this StructureAbout this Structure
1E9M is a [Single protein] structure of sequence from [Rhodobacter capsulatus] with FES as [ligand]. Structure known Active Site: FES. Full crystallographic information is available from [OCA].
ReferenceReference
Crystallization and preliminary X-ray diffraction analysis of a [2Fe-2S] ferredoxin (FdVI) from Rhodobacter capsulatus., Armengaud J, Sainz G, Jouanneau Y, Sieker LC, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):301-3. PMID:11173487 [[Category: [2fe-2s]]]
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