1sum: Difference between revisions
New page: left|200px<br /><applet load="1sum" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sum, resolution 2.00Å" /> '''Crystal structure of... |
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[[Image:1sum.gif|left|200px]]<br /><applet load="1sum" size=" | [[Image:1sum.gif|left|200px]]<br /><applet load="1sum" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1sum, resolution 2.00Å" /> | caption="1sum, resolution 2.00Å" /> | ||
'''Crystal structure of a hypothetical protein at 2.0 A resolution'''<br /> | '''Crystal structure of a hypothetical protein at 2.0 A resolution'''<br /> | ||
==Overview== | ==Overview== | ||
PhoU proteins are known to play a role in the regulation of phosphate | PhoU proteins are known to play a role in the regulation of phosphate uptake. In Thermotoga maritima, two PhoU homologues have been identified bioinformatically. Here we report the crystal structure of one of the PhoU homologues at 2.0 A resolution. The structure of the PhoU protein homologue contains a highly symmetric new structural fold composed of two repeats of a three-helix bundle. The structure unexpectedly revealed a trinuclear and a tetranuclear iron cluster that were found to be bound on the surface. Each of the two multinuclear iron clusters is coordinated by a conserved E(D)XXXD motif pair. Our structure reveals a new class of metalloprotein containing multinuclear iron clusters. The possible functional implication based on the structure are discussed. | ||
==About this Structure== | ==About this Structure== | ||
1SUM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with FE, NI, CA and PO3 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1SUM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with <scene name='pdbligand=FE:'>FE</scene>, <scene name='pdbligand=NI:'>NI</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=PO3:'>PO3</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SUM OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermotoga maritima]] | [[Category: Thermotoga maritima]] | ||
[[Category: Adams, P | [[Category: Adams, P D.]] | ||
[[Category: BSGC, Berkeley | [[Category: BSGC, Berkeley Structural Genomics Center.]] | ||
[[Category: Kim, R.]] | [[Category: Kim, R.]] | ||
[[Category: Kim, S | [[Category: Kim, S H.]] | ||
[[Category: Liu, J.]] | [[Category: Liu, J.]] | ||
[[Category: Lou, Y.]] | [[Category: Lou, Y.]] | ||
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[[Category: structure funded by nih]] | [[Category: structure funded by nih]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:05:19 2008'' | ||
Revision as of 16:05, 21 February 2008
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Crystal structure of a hypothetical protein at 2.0 A resolution
OverviewOverview
PhoU proteins are known to play a role in the regulation of phosphate uptake. In Thermotoga maritima, two PhoU homologues have been identified bioinformatically. Here we report the crystal structure of one of the PhoU homologues at 2.0 A resolution. The structure of the PhoU protein homologue contains a highly symmetric new structural fold composed of two repeats of a three-helix bundle. The structure unexpectedly revealed a trinuclear and a tetranuclear iron cluster that were found to be bound on the surface. Each of the two multinuclear iron clusters is coordinated by a conserved E(D)XXXD motif pair. Our structure reveals a new class of metalloprotein containing multinuclear iron clusters. The possible functional implication based on the structure are discussed.
About this StructureAbout this Structure
1SUM is a Single protein structure of sequence from Thermotoga maritima with , , and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of a PhoU protein homologue: a new class of metalloprotein containing multinuclear iron clusters., Liu J, Lou Y, Yokota H, Adams PD, Kim R, Kim SH, J Biol Chem. 2005 Apr 22;280(16):15960-6. Epub 2005 Feb 16. PMID:15716271
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