1sty: Difference between revisions
New page: left|200px<br /><applet load="1sty" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sty, resolution 1.67Å" /> '''THE ALPHA ANEURISM: ... |
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[[Image:1sty.jpg|left|200px]]<br /><applet load="1sty" size=" | [[Image:1sty.jpg|left|200px]]<br /><applet load="1sty" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1sty, resolution 1.67Å" /> | caption="1sty, resolution 1.67Å" /> | ||
'''THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE'''<br /> | '''THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE'''<br /> | ||
==Overview== | ==Overview== | ||
The x-ray crystal structure of a mutant of staphylococcal nuclease that | The x-ray crystal structure of a mutant of staphylococcal nuclease that contains a single glycine residue inserted in the C-terminal alpha-helix has been solved to 1.67 A resolution and refined to a crystallographic R value of 0.170. This inserted glycine residue is accommodated in the alpha-helix by formation of a previously uncharacterized bulge, which we term the alpha aneurism. A conformational search of known protein structures has identified the alpha aneurism in a number of protein families, including the histocompatibility antigens and hemoglobins. | ||
==About this Structure== | ==About this Structure== | ||
1STY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with CA and THP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Micrococcal_nuclease Micrococcal nuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.31.1 3.1.31.1] Full crystallographic information is available from [http:// | 1STY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=THP:'>THP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Micrococcal_nuclease Micrococcal nuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.31.1 3.1.31.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1STY OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
[[Category: Keefe, L | [[Category: Keefe, L J.]] | ||
[[Category: Lattman, E | [[Category: Lattman, E E.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
[[Category: THP]] | [[Category: THP]] | ||
[[Category: hydrolase (phosphoric diester)]] | [[Category: hydrolase (phosphoric diester)]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:05:07 2008'' | ||
Revision as of 16:05, 21 February 2008
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THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE
OverviewOverview
The x-ray crystal structure of a mutant of staphylococcal nuclease that contains a single glycine residue inserted in the C-terminal alpha-helix has been solved to 1.67 A resolution and refined to a crystallographic R value of 0.170. This inserted glycine residue is accommodated in the alpha-helix by formation of a previously uncharacterized bulge, which we term the alpha aneurism. A conformational search of known protein structures has identified the alpha aneurism in a number of protein families, including the histocompatibility antigens and hemoglobins.
About this StructureAbout this Structure
1STY is a Single protein structure of sequence from Staphylococcus aureus with and as ligands. Active as Micrococcal nuclease, with EC number 3.1.31.1 Full crystallographic information is available from OCA.
ReferenceReference
The alpha aneurism: a structural motif revealed in an insertion mutant of staphylococcal nuclease., Keefe LJ, Sondek J, Shortle D, Lattman EE, Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3275-9. PMID:8475069
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