1sl1: Difference between revisions

Revision as of 16:02, 21 February 2008

Binary 5' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template

OverviewOverview

Ultraviolet-induced DNA damage poses a lethal block to replication. To understand the structural basis for this, we determined crystal structures of a replicative DNA polymerase from bacteriophage T7 in complex with nucleotide substrates and a DNA template containing a cis-syn cyclobutane pyrimidine dimer (CPD). When the 3' thymine is the templating base, the CPD is rotated out of the polymerase active site and the fingers subdomain adopts an open orientation. When the 5' thymine is the templating base, the CPD lies within the polymerase active site where it base-pairs with the incoming nucleotide and the 3' base of the primer, while the fingers are in a closed conformation. These structures reveal the basis for the strong block of DNA replication that is caused by this photolesion.

About this StructureAbout this Structure

1SL1 is a Protein complex structure of sequences from Bacteriophage t7 and Escherichia coli with as ligand. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.

ReferenceReference

Nucleotide insertion opposite a cis-syn thymine dimer by a replicative DNA polymerase from bacteriophage T7., Li Y, Dutta S, Doublie S, Bdour HM, Taylor JS, Ellenberger T, Nat Struct Mol Biol. 2004 Aug;11(8):784-90. Epub 2004 Jul 4. PMID:15235589

Page seeded by OCA on Thu Feb 21 15:02:32 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1sl1.gif|left|200px]]<br /><applet load="1sl1" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1sl1.gif|left|200px]]<br /><applet load="1sl1" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1sl1, resolution 2.20&Aring;" />
 '''Binary 5' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template'''<br />
 ==Overview==
-Ultraviolet-induced DNA damage poses a lethal block to replication. To, understand the structural basis for this, we determined crystal structures, of a replicative DNA polymerase from bacteriophage T7 in complex with, nucleotide substrates and a DNA template containing a cis-syn cyclobutane, pyrimidine dimer (CPD). When the 3' thymine is the templating base, the, CPD is rotated out of the polymerase active site and the fingers subdomain, adopts an open orientation. When the 5' thymine is the templating base, the CPD lies within the polymerase active site where it base-pairs with, the incoming nucleotide and the 3' base of the primer, while the fingers, are in a closed conformation. These structures reveal the basis for the, strong block of DNA replication that is caused by this photolesion.
+Ultraviolet-induced DNA damage poses a lethal block to replication. To understand the structural basis for this, we determined crystal structures of a replicative DNA polymerase from bacteriophage T7 in complex with nucleotide substrates and a DNA template containing a cis-syn cyclobutane pyrimidine dimer (CPD). When the 3' thymine is the templating base, the CPD is rotated out of the polymerase active site and the fingers subdomain adopts an open orientation. When the 5' thymine is the templating base, the CPD lies within the polymerase active site where it base-pairs with the incoming nucleotide and the 3' base of the primer, while the fingers are in a closed conformation. These structures reveal the basis for the strong block of DNA replication that is caused by this photolesion.
 ==About this Structure==
-SL1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SL1 OCA].
+SL1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SL1 OCA].
 ==Reference==
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 [[Category: Escherichia coli]]
 [[Category: Protein complex]]
-[[Category: Bdour, H.M.]]
+[[Category: Bdour, H M.]]
 [[Category: Doublie, S.]]
 [[Category: Dutta, S.]]
 [[Category: Ellenberger, T.]]
 [[Category: Li, Y.]]
-[[Category: Taylor, J.S.]]
+[[Category: Taylor, J S.]]
 [[Category: MG]]
 [[Category: close]]
@@ Line 29: / Line 29: @@
 [[Category: thymine dimer]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:27:07 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:02:32 2008''