1sjq: Difference between revisions

[[Image:1sjq.gif|left|200px]]<br /><applet load="1sjq" size="350" color="white" frame="true" align="right" spinBox="true"  

The polypyrimidine tract binding protein (PTB) is an important regulator of alternative splicing that also affects mRNA localization, stabilization, polyadenylation, and translation. NMR structural analysis of the N-terminal half of PTB (residues 55-301) shows a canonical structure for RRM1 but reveals novel extensions to the beta strands and C terminus of RRM2 that significantly modify the beta sheet RNA binding surface. Although PTB contains four RNA recognition motifs (RRMs), it is widely held that only RRMs 3 and 4 are involved in RNA binding and that RRM2 mediates homodimerization. However, we show here not only that the RRMs 1 and 2 contribute substantially to RNA binding but also that full-length PTB is monomeric, with an elongated structure determined by X-ray solution scattering that is consistent with a linear arrangement of the constituent RRMs. These new insights into the structure and RNA binding properties of PTB suggest revised models of its mechanism of action.

1SJQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJQ OCA].  

[[Category: Cary, P D.]]

[[Category: Conte, M R.]]

[[Category: Konarev, P V.]]

[[Category: Matthews, S J.]]

[[Category: Monie, T P.]]

[[Category: Petoukhov, M V.]]

[[Category: Read, C M.]]

[[Category: Simpson, P J.]]

[[Category: Svergun, D I.]]

[[Category: Tyzack, J K.]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:02:16 2008''