1sj8: Difference between revisions

Revision as of 16:02, 21 February 2008

Crystal Structure of talin residues 482-789

OverviewOverview

The interaction between the cytoskeletal proteins talin and vinculin plays a key role in integrin-mediated cell adhesion and migration. We have determined the crystal structures of two domains from the talin rod spanning residues 482-789. Talin 482-655, which contains a vinculin-binding site (VBS), folds into a five-helix bundle whereas talin 656-789 is a four-helix bundle. We show that the VBS is composed of a hydrophobic surface spanning five turns of helix 4. All the key side chains from the VBS are buried and contribute to the hydrophobic core of the talin 482-655 fold. We demonstrate that the talin 482-655 five-helix bundle represents an inactive conformation, and mutations that disrupt the hydrophobic core or deletion of helix 5 are required to induce an active conformation in which the VBS is exposed. We also report the crystal structure of the N-terminal vinculin head domain in complex with an activated form of talin. Activation of the VBS in talin and the recruitment of vinculin may support the maturation of small integrin/talin complexes into more stable adhesions.

About this StructureAbout this Structure

1SJ8 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Activation of a vinculin-binding site in the talin rod involves rearrangement of a five-helix bundle., Papagrigoriou E, Gingras AR, Barsukov IL, Bate N, Fillingham IJ, Patel B, Frank R, Ziegler WH, Roberts GC, Critchley DR, Emsley J, EMBO J. 2004 Aug 4;23(15):2942-51. Epub 2004 Jul 22. PMID:15272303

Page seeded by OCA on Thu Feb 21 15:02:02 2008

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OCA

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-[[Image:1sj8.gif|left|200px]]<br /><applet load="1sj8" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1sj8.gif|left|200px]]<br /><applet load="1sj8" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1sj8, resolution 2.60&Aring;" />
 '''Crystal Structure of talin residues 482-789'''<br />
 ==Overview==
-The interaction between the cytoskeletal proteins talin and vinculin plays, a key role in integrin-mediated cell adhesion and migration. We have, determined the crystal structures of two domains from the talin rod, spanning residues 482-789. Talin 482-655, which contains a, vinculin-binding site (VBS), folds into a five-helix bundle whereas talin, 656-789 is a four-helix bundle. We show that the VBS is composed of a, hydrophobic surface spanning five turns of helix 4. All the key side, chains from the VBS are buried and contribute to the hydrophobic core of, the talin 482-655 fold. We demonstrate that the talin 482-655 five-helix, bundle represents an inactive conformation, and mutations that disrupt the, hydrophobic core or deletion of helix 5 are required to induce an active, conformation in which the VBS is exposed. We also report the crystal, structure of the N-terminal vinculin head domain in complex with an, activated form of talin. Activation of the VBS in talin and the, recruitment of vinculin may support the maturation of small integrin/talin, complexes into more stable adhesions.
+The interaction between the cytoskeletal proteins talin and vinculin plays a key role in integrin-mediated cell adhesion and migration. We have determined the crystal structures of two domains from the talin rod spanning residues 482-789. Talin 482-655, which contains a vinculin-binding site (VBS), folds into a five-helix bundle whereas talin 656-789 is a four-helix bundle. We show that the VBS is composed of a hydrophobic surface spanning five turns of helix 4. All the key side chains from the VBS are buried and contribute to the hydrophobic core of the talin 482-655 fold. We demonstrate that the talin 482-655 five-helix bundle represents an inactive conformation, and mutations that disrupt the hydrophobic core or deletion of helix 5 are required to induce an active conformation in which the VBS is exposed. We also report the crystal structure of the N-terminal vinculin head domain in complex with an activated form of talin. Activation of the VBS in talin and the recruitment of vinculin may support the maturation of small integrin/talin complexes into more stable adhesions.
 ==About this Structure==
-SJ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SJ8 OCA].
+SJ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJ8 OCA].
 ==Reference==
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 [[Category: Mus musculus]]
 [[Category: Single protein]]
-[[Category: Barsukov, I.L.]]
+[[Category: Barsukov, I L.]]
-[[Category: Critchley, D.R.]]
+[[Category: Critchley, D R.]]
 [[Category: Emsley, J.]]
-[[Category: Gingras, A.R.]]
+[[Category: Gingras, A R.]]
 [[Category: Papagrigoriou, E.]]
 [[Category: structural protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:25:08 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:02:02 2008''