1si8: Difference between revisions
New page: left|200px<br /><applet load="1si8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1si8, resolution 2.30Å" /> '''Crystal structure of... |
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[[Image:1si8.jpg|left|200px]]<br /><applet load="1si8" size=" | [[Image:1si8.jpg|left|200px]]<br /><applet load="1si8" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1si8, resolution 2.30Å" /> | caption="1si8, resolution 2.30Å" /> | ||
'''Crystal structure of E. faecalis catalase'''<br /> | '''Crystal structure of E. faecalis catalase'''<br /> | ||
==Overview== | ==Overview== | ||
Enterococcus faecalis haem catalase was crystallized using lithium sulfate | Enterococcus faecalis haem catalase was crystallized using lithium sulfate at neutral pH. The crystals belong to space group R3, with unit-cell parameters a = b = 236.9, c = 198.1 A. The three-dimensional structure was determined by molecular replacement using a subunit of the Proteus mirabilis catalase structure. It was refined against 2.3 A synchrotron data to a free R factor of 21.8%. Like other catalases, the E. faecalis catalase is a homotetramer with a fold and structure similar to those of its structurally closest relative P. mirabilis. The solvent structure in the active site is identical in the four subunits but differs from that found in other catalases. The structural consequences of the Ramachandran outlier Ser196 are discussed. | ||
==About this Structure== | ==About this Structure== | ||
1SI8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis] with SO4, CL and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] Full crystallographic information is available from [http:// | 1SI8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SI8 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Brugna, M.]] | [[Category: Brugna, M.]] | ||
[[Category: Hakansson, K | [[Category: Hakansson, K O.]] | ||
[[Category: Tasse, L.]] | [[Category: Tasse, L.]] | ||
[[Category: CL]] | [[Category: CL]] | ||
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[[Category: n-terminal arm; anti-parallel beta-barrel; wrapping region; c-terminal helical region; tetramer; heme group]] | [[Category: n-terminal arm; anti-parallel beta-barrel; wrapping region; c-terminal helical region; tetramer; heme group]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:01:46 2008'' | ||
Revision as of 16:01, 21 February 2008
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Crystal structure of E. faecalis catalase
OverviewOverview
Enterococcus faecalis haem catalase was crystallized using lithium sulfate at neutral pH. The crystals belong to space group R3, with unit-cell parameters a = b = 236.9, c = 198.1 A. The three-dimensional structure was determined by molecular replacement using a subunit of the Proteus mirabilis catalase structure. It was refined against 2.3 A synchrotron data to a free R factor of 21.8%. Like other catalases, the E. faecalis catalase is a homotetramer with a fold and structure similar to those of its structurally closest relative P. mirabilis. The solvent structure in the active site is identical in the four subunits but differs from that found in other catalases. The structural consequences of the Ramachandran outlier Ser196 are discussed.
About this StructureAbout this Structure
1SI8 is a Single protein structure of sequence from Enterococcus faecalis with , and as ligands. Active as Catalase, with EC number 1.11.1.6 Full crystallographic information is available from OCA.
ReferenceReference
The three-dimensional structure of catalase from Enterococcus faecalis., Hakansson KO, Brugna M, Tasse L, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1374-80. Epub 2004, Jul 21. PMID:15272159
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