1sdo: Difference between revisions
New page: left|200px<br /><applet load="1sdo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sdo, resolution 1.85Å" /> '''Crystal Structure of... |
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[[Image:1sdo.jpg|left|200px]]<br /><applet load="1sdo" size=" | [[Image:1sdo.jpg|left|200px]]<br /><applet load="1sdo" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1sdo, resolution 1.85Å" /> | caption="1sdo, resolution 1.85Å" /> | ||
'''Crystal Structure of Restriction Endonuclease BstYI'''<br /> | '''Crystal Structure of Restriction Endonuclease BstYI'''<br /> | ||
==Overview== | ==Overview== | ||
We report here the structure of BstYI, an "intermediate" type II | We report here the structure of BstYI, an "intermediate" type II restriction endonuclease with overlapping sequence specificities to BamHI and BglII. BstYI, a thermophilic endonuclease, recognizes and cleaves the degenerate hexanucleotide sequence 5'-RGATCY-3' (where R=A or G and Y=C or T), cleaving DNA after the 5'-R on each strand to produce four-base (5') staggered ends. The crystal structure of free BstYI was solved at 1.85A resolution by multi-wavelength anomalous dispersion (MAD) phasing. Comparison with BamHI and BglII reveals a strong structural consensus between all three enzymes mapping to the alpha/beta core domain and residues involved in catalysis. Unexpectedly, BstYI also contains an additional "arm" substructure outside of the core protein, which enables the enzyme to adopt a more compact, intertwined dimer structure compared with BamHI and BglII. This arm substructure may underlie the thermostability of BstYI. We identify putative DNA recognition residues and speculate as to how this enzyme achieves a "relaxed" DNA specificity. | ||
==About this Structure== | ==About this Structure== | ||
1SDO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http:// | 1SDO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SDO OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Type II site-specific deoxyribonuclease]] | [[Category: Type II site-specific deoxyribonuclease]] | ||
[[Category: Aggarwal, A | [[Category: Aggarwal, A K.]] | ||
[[Category: Edwards, T | [[Category: Edwards, T A.]] | ||
[[Category: Escalante, C | [[Category: Escalante, C R.]] | ||
[[Category: Samuelson, J | [[Category: Samuelson, J C.]] | ||
[[Category: Townson, S | [[Category: Townson, S A.]] | ||
[[Category: Vanamee, E | [[Category: Vanamee, E S.]] | ||
[[Category: Xu, S | [[Category: Xu, S Y.]] | ||
[[Category: restriction endonuclease]] | [[Category: restriction endonuclease]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:00:25 2008'' | ||
Revision as of 16:00, 21 February 2008
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Crystal Structure of Restriction Endonuclease BstYI
OverviewOverview
We report here the structure of BstYI, an "intermediate" type II restriction endonuclease with overlapping sequence specificities to BamHI and BglII. BstYI, a thermophilic endonuclease, recognizes and cleaves the degenerate hexanucleotide sequence 5'-RGATCY-3' (where R=A or G and Y=C or T), cleaving DNA after the 5'-R on each strand to produce four-base (5') staggered ends. The crystal structure of free BstYI was solved at 1.85A resolution by multi-wavelength anomalous dispersion (MAD) phasing. Comparison with BamHI and BglII reveals a strong structural consensus between all three enzymes mapping to the alpha/beta core domain and residues involved in catalysis. Unexpectedly, BstYI also contains an additional "arm" substructure outside of the core protein, which enables the enzyme to adopt a more compact, intertwined dimer structure compared with BamHI and BglII. This arm substructure may underlie the thermostability of BstYI. We identify putative DNA recognition residues and speculate as to how this enzyme achieves a "relaxed" DNA specificity.
About this StructureAbout this Structure
1SDO is a Single protein structure of sequence from Geobacillus stearothermophilus. Active as Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of BstYI at 1.85A resolution: a thermophilic restriction endonuclease with overlapping specificities to BamHI and BglII., Townson SA, Samuelson JC, Vanamee ES, Edwards TA, Escalante CR, Xu SY, Aggarwal AK, J Mol Biol. 2004 May 7;338(4):725-33. PMID:15099740
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