1s4u: Difference between revisions
New page: left|200px<br /><applet load="1s4u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s4u, resolution 2.10Å" /> '''Crystal Structure an... |
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[[Image:1s4u.jpg|left|200px]]<br /><applet load="1s4u" size=" | [[Image:1s4u.jpg|left|200px]]<br /><applet load="1s4u" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1s4u, resolution 2.10Å" /> | caption="1s4u, resolution 2.10Å" /> | ||
'''Crystal Structure analysis of the beta-propeller protein Ski8p'''<br /> | '''Crystal Structure analysis of the beta-propeller protein Ski8p'''<br /> | ||
==Overview== | ==Overview== | ||
Ski8p is a WD-repeat protein with an essential role for the Ski complex | Ski8p is a WD-repeat protein with an essential role for the Ski complex assembly in an exosome-dependent 3'-to-5' mRNA decay. In addition, Ski8p is involved in meiotic recombination by interacting with Spo11p protein. We have determined the crystal structure of Ski8p from Saccharomyces cerevisiae at 2.2 A resolution. The structure reveals that Ski8p folds into a seven-bladed beta propeller. Mapping sequence conservation and hydrophobicities of amino acids on the molecular surface of Ski8p reveals a prominent site on the top surface of the beta propeller, which is most likely involved in mediating interactions of Ski8p with Ski3p and Spo11p. Mutagenesis combined with yeast two-hybrid and GST pull-down assays identified the top surface of the beta propeller as being required for Ski8p binding to Ski3p and Spo11p. The functional implications for Ski8p function in both mRNA decay and meiotic recombination are discussed. | ||
==About this Structure== | ==About this Structure== | ||
1S4U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | 1S4U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: wd repeat]] | [[Category: wd repeat]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:57:56 2008'' | ||
Revision as of 15:58, 21 February 2008
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Crystal Structure analysis of the beta-propeller protein Ski8p
OverviewOverview
Ski8p is a WD-repeat protein with an essential role for the Ski complex assembly in an exosome-dependent 3'-to-5' mRNA decay. In addition, Ski8p is involved in meiotic recombination by interacting with Spo11p protein. We have determined the crystal structure of Ski8p from Saccharomyces cerevisiae at 2.2 A resolution. The structure reveals that Ski8p folds into a seven-bladed beta propeller. Mapping sequence conservation and hydrophobicities of amino acids on the molecular surface of Ski8p reveals a prominent site on the top surface of the beta propeller, which is most likely involved in mediating interactions of Ski8p with Ski3p and Spo11p. Mutagenesis combined with yeast two-hybrid and GST pull-down assays identified the top surface of the beta propeller as being required for Ski8p binding to Ski3p and Spo11p. The functional implications for Ski8p function in both mRNA decay and meiotic recombination are discussed.
About this StructureAbout this Structure
1S4U is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination., Cheng Z, Liu Y, Wang C, Parker R, Song H, Protein Sci. 2004 Oct;13(10):2673-84. Epub 2004 Aug 31. PMID:15340168
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