1s03: Difference between revisions
New page: left|200px<br /><applet load="1s03" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s03, resolution 2.70Å" /> '''The Structure of a R... |
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[[Image:1s03.gif|left|200px]]<br /><applet load="1s03" size=" | [[Image:1s03.gif|left|200px]]<br /><applet load="1s03" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1s03, resolution 2.70Å" /> | caption="1s03, resolution 2.70Å" /> | ||
'''The Structure of a Ribosomal Protein S8/spc Operon mRNA Complex'''<br /> | '''The Structure of a Ribosomal Protein S8/spc Operon mRNA Complex'''<br /> | ||
==Overview== | ==Overview== | ||
In bacteria, translation of all the ribosomal protein cistrons in the spc | In bacteria, translation of all the ribosomal protein cistrons in the spc operon mRNA is repressed by the binding of the product of one of them, S8, to an internal sequence at the 5' end of the L5 cistron. The way in which the first two genes of the spc operon are regulated, retroregulation, is mechanistically distinct from translational repression by S8 of the genes from L5 onward. A 2.8 A resolution crystal structure has been obtained of Escherichia coli S8 bound to this site. Despite sequence differences, the structure of this complex is almost identical to that of the S8/helix 21 complex seen in the small ribosomal subunit, consistent with the hypothesis that autogenous regulation of ribosomal protein synthesis results from conformational similarities between mRNAs and rRNAs. S8 binding must repress the translation of its own mRNA by inhibiting the formation of a ribosomal initiation complex at the start of the L5 cistron. | ||
==About this Structure== | ==About this Structure== | ||
1S03 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1S03 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S03 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Merianos, H | [[Category: Merianos, H J.]] | ||
[[Category: Moore, P | [[Category: Moore, P B.]] | ||
[[Category: Wang, J.]] | [[Category: Wang, J.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: spc operon]] | [[Category: spc operon]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:29 2008'' | ||
Revision as of 15:56, 21 February 2008
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The Structure of a Ribosomal Protein S8/spc Operon mRNA Complex
OverviewOverview
In bacteria, translation of all the ribosomal protein cistrons in the spc operon mRNA is repressed by the binding of the product of one of them, S8, to an internal sequence at the 5' end of the L5 cistron. The way in which the first two genes of the spc operon are regulated, retroregulation, is mechanistically distinct from translational repression by S8 of the genes from L5 onward. A 2.8 A resolution crystal structure has been obtained of Escherichia coli S8 bound to this site. Despite sequence differences, the structure of this complex is almost identical to that of the S8/helix 21 complex seen in the small ribosomal subunit, consistent with the hypothesis that autogenous regulation of ribosomal protein synthesis results from conformational similarities between mRNAs and rRNAs. S8 binding must repress the translation of its own mRNA by inhibiting the formation of a ribosomal initiation complex at the start of the L5 cistron.
About this StructureAbout this Structure
1S03 is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
The structure of a ribosomal protein S8/spc operon mRNA complex., Merianos HJ, Wang J, Moore PB, RNA. 2004 Jun;10(6):954-64. PMID:15146079
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