1rwj: Difference between revisions
New page: left|200px<br /><applet load="1rwj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rwj, resolution 1.70Å" /> '''c7-type three-heme c... |
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[[Image:1rwj.gif|left|200px]]<br /><applet load="1rwj" size=" | [[Image:1rwj.gif|left|200px]]<br /><applet load="1rwj" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1rwj, resolution 1.70Å" /> | caption="1rwj, resolution 1.70Å" /> | ||
'''c7-type three-heme cytochrome domain'''<br /> | '''c7-type three-heme cytochrome domain'''<br /> | ||
==Overview== | ==Overview== | ||
The structure of a novel c(7)-type cytochrome domain that has two | The structure of a novel c(7)-type cytochrome domain that has two bishistidine coordinated hemes and one heme with histidine, methionine coordination (where the sixth ligand is a methionine residue) was determined at 1.7 A resolution. This domain is a representative of domains that form three polymers encoded by the Geobacter sulfurreducens genome. Two of these polymers consist of four and one protein of nine c(7)-type domains with a total of 12 and 27 hemes, respectively. Four individual domains (termed A, B, C, and D) from one such multiheme cytochrome c (ORF03300) were cloned and expressed in Escherichia coli. The domain C produced diffraction quality crystals from 2.4 M sodium malonate (pH 7). The structure was solved by MAD method and refined to an R-factor of 19.5% and R-free of 21.8%. Unlike the two c(7) molecules with known structures, one from G. sulfurreducens (PpcA) and one from Desulfuromonas acetoxidans where all three hemes are bishistidine coordinated, this domain contains a heme which is coordinated by a methionine and a histidine residue. As a result, the corresponding heme could have a higher potential than the other two hemes. The apparent midpoint reduction potential, E(app), of domain C is -105 mV, 50 mV higher than that of PpcA. | ||
==About this Structure== | ==About this Structure== | ||
1RWJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1RWJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens] with <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWJ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Geobacter sulfurreducens]] | [[Category: Geobacter sulfurreducens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Duke, N | [[Category: Duke, N E.C.]] | ||
[[Category: Erickson, J.]] | [[Category: Erickson, J.]] | ||
[[Category: Londer, Y | [[Category: Londer, Y Y.]] | ||
[[Category: Pessanha, M.]] | [[Category: Pessanha, M.]] | ||
[[Category: Pokkuluri, P | [[Category: Pokkuluri, P R.]] | ||
[[Category: Salgueiro, C | [[Category: Salgueiro, C A.]] | ||
[[Category: Schiffer, M.]] | [[Category: Schiffer, M.]] | ||
[[Category: HEM]] | [[Category: HEM]] | ||
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[[Category: multiheme cytochrome c]] | [[Category: multiheme cytochrome c]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:15 2008'' | ||
Revision as of 15:55, 21 February 2008
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c7-type three-heme cytochrome domain
OverviewOverview
The structure of a novel c(7)-type cytochrome domain that has two bishistidine coordinated hemes and one heme with histidine, methionine coordination (where the sixth ligand is a methionine residue) was determined at 1.7 A resolution. This domain is a representative of domains that form three polymers encoded by the Geobacter sulfurreducens genome. Two of these polymers consist of four and one protein of nine c(7)-type domains with a total of 12 and 27 hemes, respectively. Four individual domains (termed A, B, C, and D) from one such multiheme cytochrome c (ORF03300) were cloned and expressed in Escherichia coli. The domain C produced diffraction quality crystals from 2.4 M sodium malonate (pH 7). The structure was solved by MAD method and refined to an R-factor of 19.5% and R-free of 21.8%. Unlike the two c(7) molecules with known structures, one from G. sulfurreducens (PpcA) and one from Desulfuromonas acetoxidans where all three hemes are bishistidine coordinated, this domain contains a heme which is coordinated by a methionine and a histidine residue. As a result, the corresponding heme could have a higher potential than the other two hemes. The apparent midpoint reduction potential, E(app), of domain C is -105 mV, 50 mV higher than that of PpcA.
About this StructureAbout this Structure
1RWJ is a Single protein structure of sequence from Geobacter sulfurreducens with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Structure of a novel c7-type three-heme cytochrome domain from a multidomain cytochrome c polymer., Pokkuluri PR, Londer YY, Duke NE, Erickson J, Pessanha M, Salgueiro CA, Schiffer M, Protein Sci. 2004 Jun;13(6):1684-92. Epub 2004 May 7. PMID:15133162
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