1rc7: Difference between revisions

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Crystal structure of RNase III Mutant E110K from Aquifex Aeolicus complexed with ds-RNA at 2.15 Angstrom Resolution

OverviewOverview

Ribonuclease III (RNase III) represents a family of double-stranded RNA (dsRNA) endonucleases. The simplest bacterial enzyme contains an endonuclease domain (endoND) and a dsRNA binding domain (dsRBD). RNase III can affect RNA structure and gene expression in either of two ways: as a dsRNA-processing enzyme that cleaves dsRNA, or as a dsRNA binding protein that binds but does not cleave dsRNA. We previously determined the endoND structure of Aquifex aeolicus RNase III (Aa-RNase III) and modeled a catalytic complex of full-length Aa-RNase III with dsRNA. Here, we present the crystal structure of Aa-RNase III in complex with dsRNA, revealing a noncatalytic assembly. The major differences between the two functional forms of RNase III.dsRNA are the conformation of the protein and the orientation and location of dsRNA. The flexibility of a 7 residue linker between the endoND and dsRBD enables the transition between these two forms.

About this StructureAbout this Structure

1RC7 is a Single protein structure of sequence from Aquifex aeolicus with as ligand. Active as Ribonuclease III, with EC number 3.1.26.3 Full crystallographic information is available from OCA.

ReferenceReference

Noncatalytic assembly of ribonuclease III with double-stranded RNA., Blaszczyk J, Gan J, Tropea JE, Court DL, Waugh DS, Ji X, Structure. 2004 Mar;12(3):457-66. PMID:15016361

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-[[Image:1rc7.gif|left|200px]]<br /><applet load="1rc7" size="450" color="white" frame="true" align="right" spinBox="true"
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 caption="1rc7, resolution 2.15&Aring;" />
 '''Crystal structure of RNase III Mutant E110K from Aquifex Aeolicus complexed with ds-RNA at 2.15 Angstrom Resolution'''<br />
 ==Overview==
-Ribonuclease III (RNase III) represents a family of double-stranded RNA, (dsRNA) endonucleases. The simplest bacterial enzyme contains an, endonuclease domain (endoND) and a dsRNA binding domain (dsRBD). RNase III, can affect RNA structure and gene expression in either of two ways: as a, dsRNA-processing enzyme that cleaves dsRNA, or as a dsRNA binding protein, that binds but does not cleave dsRNA. We previously determined the endoND, structure of Aquifex aeolicus RNase III (Aa-RNase III) and modeled a, catalytic complex of full-length Aa-RNase III with dsRNA. Here, we present, the crystal structure of Aa-RNase III in complex with dsRNA, revealing a, noncatalytic assembly. The major differences between the two functional, forms of RNase III.dsRNA are the conformation of the protein and the, orientation and location of dsRNA. The flexibility of a 7 residue linker, between the endoND and dsRBD enables the transition between these two, forms.
+Ribonuclease III (RNase III) represents a family of double-stranded RNA (dsRNA) endonucleases. The simplest bacterial enzyme contains an endonuclease domain (endoND) and a dsRNA binding domain (dsRBD). RNase III can affect RNA structure and gene expression in either of two ways: as a dsRNA-processing enzyme that cleaves dsRNA, or as a dsRNA binding protein that binds but does not cleave dsRNA. We previously determined the endoND structure of Aquifex aeolicus RNase III (Aa-RNase III) and modeled a catalytic complex of full-length Aa-RNase III with dsRNA. Here, we present the crystal structure of Aa-RNase III in complex with dsRNA, revealing a noncatalytic assembly. The major differences between the two functional forms of RNase III.dsRNA are the conformation of the protein and the orientation and location of dsRNA. The flexibility of a 7 residue linker between the endoND and dsRBD enables the transition between these two forms.
 ==About this Structure==
-RC7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with TRS as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RC7 OCA].
+RC7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RC7 OCA].
 ==Reference==
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 [[Category: rna interference]]
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