Structure of E. coli DnaC helicase loader: Difference between revisions
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The following analysis utilizes the homology model templated on [[2qgz]]. The model templated on [[3ecc]] is very similar, with identical sequence-to-structure registration (not shown). When the two homology models are structurally aligned, 115 alpha carbon atoms can be aligned with RMS deviation of 1.4 Å (not shown). | The following analysis utilizes the homology model templated on [[2qgz]]. The model templated on [[3ecc]] is very similar, with identical sequence-to-structure registration (not shown). When the two homology models are structurally aligned, 115 alpha carbon atoms can be aligned with RMS deviation of 1.4 Å (not shown). | ||
The homology model (<scene name='User:Eric_Martz/Sandbox_4/Dnac_model_from_2ggz_a/8'>restore initial scene</scene>) represents 75% of the full length ''E. coli'' DnaC sequence, omitting 54 N-terminal residues | The homology model (<scene name='User:Eric_Martz/Sandbox_4/Dnac_model_from_2ggz_a/8'>restore initial scene</scene>) represents 75% of the full length ''E. coli'' DnaC sequence, omitting 54 N-terminal residues, and 8 C-terminal residues. Note that the N-terminal 76 residues are predicted to be intrinsically unfolded (see [[#Intrinsically Unstructured N-Terminus|above]]). Several surface loops in the model (shown translucent white) have high uncertainty, since these are missing in the template ([[#Homology Model Construction|see below]]). Note that the homology model is somewhat unreliable about which residues are actually on the surface vs. largely buried, and hence the conclusions below are tentative. | ||
{{Template:ColorKey_Amino2CarboxyRainbow}} | {{Template:ColorKey_Amino2CarboxyRainbow}} | ||