1r7r: Difference between revisions

Revision as of 15:48, 21 February 2008

The crystal structure of murine p97/VCP at 3.6A

OverviewOverview

p97/VCP is a member of the AAA ATPase family and has roles in both membrane fusion and ubiquitin dependent protein degradation. Here, we present a 3.6A crystal structure of murine p97 in which D2 domain has been modelled as poly-alanine and the remaining approximately 100 residues are absent. The resulting structure illustrates a head-to-tail packing arrangement of the two p97 AAA domains in a natural hexameric state with D1 ADP bound and D2 nucleotide free. The head-to-tail packing arrangement observed in this structure is in contrast to our previously predicted tail-to-tail packing model. The linker between the D1 and D2 domains is partially disordered, suggesting a flexible nature. Normal mode analysis of the crystal structure suggests anti-correlated motions and distinct conformational states of the two AAA domains.

About this StructureAbout this Structure

1R7R is a Single protein structure of sequence from Mus musculus with as ligand. The following page contains interesting information on the relation of 1R7R with [AAA+ Proteases]. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of murine p97/VCP at 3.6A., Huyton T, Pye VE, Briggs LC, Flynn TC, Beuron F, Kondo H, Ma J, Zhang X, Freemont PS, J Struct Biol. 2003 Dec;144(3):337-48. PMID:14643202

Page seeded by OCA on Thu Feb 21 14:48:08 2008

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OCA

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-[[Image:1r7r.gif|left|200px]]<br />
+[[Image:1r7r.gif|left|200px]]<br /><applet load="1r7r" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1r7r" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1r7r, resolution 3.60&Aring;" />
 '''The crystal structure of murine p97/VCP at 3.6A'''<br />
 ==Overview==
-p97/VCP is a member of the AAA ATPase family and has roles in both, membrane fusion and ubiquitin dependent protein degradation. Here, we, present a 3.6A crystal structure of murine p97 in which D2 domain has been, modelled as poly-alanine and the remaining approximately 100 residues are, absent. The resulting structure illustrates a head-to-tail packing, arrangement of the two p97 AAA domains in a natural hexameric state with, D1 ADP bound and D2 nucleotide free. The head-to-tail packing arrangement, observed in this structure is in contrast to our previously predicted, tail-to-tail packing model. The linker between the D1 and D2 domains is, partially disordered, suggesting a flexible nature. Normal mode analysis, of the crystal structure suggests anti-correlated motions and distinct, conformational states of the two AAA domains.
+p97/VCP is a member of the AAA ATPase family and has roles in both membrane fusion and ubiquitin dependent protein degradation. Here, we present a 3.6A crystal structure of murine p97 in which D2 domain has been modelled as poly-alanine and the remaining approximately 100 residues are absent. The resulting structure illustrates a head-to-tail packing arrangement of the two p97 AAA domains in a natural hexameric state with D1 ADP bound and D2 nucleotide free. The head-to-tail packing arrangement observed in this structure is in contrast to our previously predicted tail-to-tail packing model. The linker between the D1 and D2 domains is partially disordered, suggesting a flexible nature. Normal mode analysis of the crystal structure suggests anti-correlated motions and distinct conformational states of the two AAA domains.
 ==About this Structure==
-R7R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1R7R with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R7R OCA].
+R7R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1R7R with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R7R OCA].
 ==Reference==
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 [[Category: Single protein]]
 [[Category: Beuron, F.]]
-[[Category: Briggs, L.C.]]
+[[Category: Briggs, L C.]]
-[[Category: Flynn, T.C.]]
+[[Category: Flynn, T C.]]
-[[Category: Freemont, P.S.]]
+[[Category: Freemont, P S.]]
 [[Category: Huyton, T.]]
 [[Category: Kondo, H.]]
 [[Category: Ma, J.]]
-[[Category: Pye, V.E.]]
+[[Category: Pye, V E.]]
 [[Category: Zhang, X.]]
 [[Category: ADP]]
@@ Line 30: / Line 29: @@
 [[Category: vcp]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:05:22 2007''
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