1r0d: Difference between revisions

Revision as of 15:45, 21 February 2008

HIP1R THATCH DOMAIN CORE

OverviewOverview

Huntingtin-interacting protein-1 related (HIP1R) has a crucial protein-trafficking role, mediating associations between actin and clathrin-coated structures at the plasma membrane and trans-Golgi network. Here, we characterize the F-actin-binding region of HIP1R, termed the talin-HIP1/R/Sla2p actin-tethering C-terminal homology (THATCH) domain. The 1.9-A crystal structure of the human HIP1R THATCH core reveals a large sequence-conserved surface patch created primarily by residues from the third and fourth helices of a unique five-helix bundle. Point mutations of seven contiguous patch residues produced significant decreases in F-actin binding. We also show that THATCH domains have a conserved C-terminal latch capable of oligomerizing the core, thereby modulating F-actin engagement. Collectively, these results establish a framework for investigating the links between endocytosis and actin dynamics mediated by THATCH domain-containing proteins.

About this StructureAbout this Structure

1R0D is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural definition of the F-actin-binding THATCH domain from HIP1R., Brett TJ, Legendre-Guillemin V, McPherson PS, Fremont DH, Nat Struct Mol Biol. 2006 Feb;13(2):121-30. Epub 2006 Jan 15. PMID:16415883

Page seeded by OCA on Thu Feb 21 14:45:39 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1r0d.gif|left|200px]]<br />
+[[Image:1r0d.gif|left|200px]]<br /><applet load="1r0d" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1r0d" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1r0d, resolution 1.90&Aring;" />
 '''HIP1R THATCH DOMAIN CORE'''<br />
 ==Overview==
-Huntingtin-interacting protein-1 related (HIP1R) has a crucial, protein-trafficking role, mediating associations between actin and, clathrin-coated structures at the plasma membrane and trans-Golgi network., Here, we characterize the F-actin-binding region of HIP1R, termed the, talin-HIP1/R/Sla2p actin-tethering C-terminal homology (THATCH) domain., The 1.9-A crystal structure of the human HIP1R THATCH core reveals a large, sequence-conserved surface patch created primarily by residues from the, third and fourth helices of a unique five-helix bundle. Point mutations of, seven contiguous patch residues produced significant decreases in F-actin, binding. We also show that THATCH domains have a conserved C-terminal, latch capable of oligomerizing the core, thereby modulating F-actin, engagement. Collectively, these results establish a framework for, investigating the links between endocytosis and actin dynamics mediated by, THATCH domain-containing proteins.
+Huntingtin-interacting protein-1 related (HIP1R) has a crucial protein-trafficking role, mediating associations between actin and clathrin-coated structures at the plasma membrane and trans-Golgi network. Here, we characterize the F-actin-binding region of HIP1R, termed the talin-HIP1/R/Sla2p actin-tethering C-terminal homology (THATCH) domain. The 1.9-A crystal structure of the human HIP1R THATCH core reveals a large sequence-conserved surface patch created primarily by residues from the third and fourth helices of a unique five-helix bundle. Point mutations of seven contiguous patch residues produced significant decreases in F-actin binding. We also show that THATCH domains have a conserved C-terminal latch capable of oligomerizing the core, thereby modulating F-actin engagement. Collectively, these results establish a framework for investigating the links between endocytosis and actin dynamics mediated by THATCH domain-containing proteins.
 ==About this Structure==
-R0D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R0D OCA].
+R0D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R0D OCA].
 ==Reference==
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 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Brett, T.J.]]
+[[Category: Brett, T J.]]
-[[Category: Fremont, D.H.]]
+[[Category: Fremont, D H.]]
-[[Category: MCSG, Midwest.Center.for.Structural.Genomics.]]
+[[Category: MCSG, Midwest Center for Structural Genomics.]]
 [[Category: actin-binding]]
 [[Category: endocytosis]]
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 [[Category: structural genomics]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:58:11 2007''
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