1qxo: Difference between revisions
New page: left|200px<br /><applet load="1qxo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qxo, resolution 2.0Å" /> '''Crystal structure of ... |
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[[Image:1qxo.jpg|left|200px]]<br /><applet load="1qxo" size=" | [[Image:1qxo.jpg|left|200px]]<br /><applet load="1qxo" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1qxo, resolution 2.0Å" /> | caption="1qxo, resolution 2.0Å" /> | ||
'''Crystal structure of Chorismate synthase complexed with oxidized FMN and EPSP'''<br /> | '''Crystal structure of Chorismate synthase complexed with oxidized FMN and EPSP'''<br /> | ||
==Overview== | ==Overview== | ||
The crystal structure of chorismate synthase (CS) from Streptococcus | The crystal structure of chorismate synthase (CS) from Streptococcus pneumoniae has been solved to 2.0 A resolution in the presence of flavin mononucleotide (FMN) and the substrate 5-enolpyruvyl-3-shikimate phosphate (EPSP). CS catalyses the final step of the shikimate pathway and is a potential therapeutic target for the rational design of novel antibacterials, antifungals, antiprotozoals, and herbicides. CS is a tetramer with the monomer possessing a novel beta-alpha-beta fold. The interactions between the enzyme, cofactor, and substrate reveal the structural reasons underlying the unique catalytic mechanism and identify the amino acids involved. This structure provides the essential initial information necessary for the generation of novel anti-infective compounds by a structure-guided medicinal chemistry approach. | ||
==About this Structure== | ==About this Structure== | ||
1QXO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] with NCO, EDO, FMN and EPS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chorismate_synthase Chorismate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.5 4.2.3.5] Full crystallographic information is available from [http:// | 1QXO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] with <scene name='pdbligand=NCO:'>NCO</scene>, <scene name='pdbligand=EDO:'>EDO</scene>, <scene name='pdbligand=FMN:'>FMN</scene> and <scene name='pdbligand=EPS:'>EPS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chorismate_synthase Chorismate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.5 4.2.3.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXO OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: shikimate]] | [[Category: shikimate]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:44:54 2008'' | ||
Revision as of 15:44, 21 February 2008
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Crystal structure of Chorismate synthase complexed with oxidized FMN and EPSP
OverviewOverview
The crystal structure of chorismate synthase (CS) from Streptococcus pneumoniae has been solved to 2.0 A resolution in the presence of flavin mononucleotide (FMN) and the substrate 5-enolpyruvyl-3-shikimate phosphate (EPSP). CS catalyses the final step of the shikimate pathway and is a potential therapeutic target for the rational design of novel antibacterials, antifungals, antiprotozoals, and herbicides. CS is a tetramer with the monomer possessing a novel beta-alpha-beta fold. The interactions between the enzyme, cofactor, and substrate reveal the structural reasons underlying the unique catalytic mechanism and identify the amino acids involved. This structure provides the essential initial information necessary for the generation of novel anti-infective compounds by a structure-guided medicinal chemistry approach.
About this StructureAbout this Structure
1QXO is a Single protein structure of sequence from Streptococcus pneumoniae with , , and as ligands. Active as Chorismate synthase, with EC number 4.2.3.5 Full crystallographic information is available from OCA.
ReferenceReference
The structure of chorismate synthase reveals a novel flavin binding site fundamental to a unique chemical reaction., Maclean J, Ali S, Structure. 2003 Dec;11(12):1499-511. PMID:14656434
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