1qwz: Difference between revisions

Revision as of 15:44, 21 February 2008

Crystal structure of Sortase B from S. aureus complexed with MTSET

OverviewOverview

Many surface proteins of Gram-positive bacteria, which play important roles during the pathogenesis of human infections, are anchored to the cell wall envelope by a mechanism requiring sortases. Sortase B, a cysteine transpeptidase from Staphylococcus aureus, cleaves the C-terminal sorting signal of IsdC at the NPQTN motif and tethers the polypeptide to the pentaglycine cell wall cross-bridge. During catalysis, the active site cysteine of sortase and the cleaved substrate form an acyl intermediate, which is then resolved by the amino group of pentaglycine cross-bridges. We report here the crystal structures of SrtBDeltaN30 in complex with two active site inhibitors, MTSET and E64, and with the cell wall substrate analog tripleglycine. These structures reveal, for the first time, the active site disposition and the unique Cys-Arg catalytic machinery of the cysteine transpeptidase, and they also provide useful information for the future design of anti-infective agents against sortases.

About this StructureAbout this Structure

1QWZ is a Single protein structure of sequence from Staphylococcus aureus with , and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

The structure of sortase B, a cysteine transpeptidase that tethers surface protein to the Staphylococcus aureus cell wall., Zong Y, Mazmanian SK, Schneewind O, Narayana SV, Structure. 2004 Jan;12(1):105-12. PMID:14725770

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OCA

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-[[Image:1qwz.jpg|left|200px]]<br /><applet load="1qwz" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1qwz.jpg|left|200px]]<br /><applet load="1qwz" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1qwz, resolution 1.75&Aring;" />
 '''Crystal structure of Sortase B from S. aureus complexed with MTSET'''<br />
 ==Overview==
-Many surface proteins of Gram-positive bacteria, which play important, roles during the pathogenesis of human infections, are anchored to the, cell wall envelope by a mechanism requiring sortases. Sortase B, a, cysteine transpeptidase from Staphylococcus aureus, cleaves the C-terminal, sorting signal of IsdC at the NPQTN motif and tethers the polypeptide to, the pentaglycine cell wall cross-bridge. During catalysis, the active site, cysteine of sortase and the cleaved substrate form an acyl intermediate, which is then resolved by the amino group of pentaglycine cross-bridges., We report here the crystal structures of SrtBDeltaN30 in complex with two, active site inhibitors, MTSET and E64, and with the cell wall substrate, analog tripleglycine. These structures reveal, for the first time, the, active site disposition and the unique Cys-Arg catalytic machinery of the, cysteine transpeptidase, and they also provide useful information for the, future design of anti-infective agents against sortases.
+Many surface proteins of Gram-positive bacteria, which play important roles during the pathogenesis of human infections, are anchored to the cell wall envelope by a mechanism requiring sortases. Sortase B, a cysteine transpeptidase from Staphylococcus aureus, cleaves the C-terminal sorting signal of IsdC at the NPQTN motif and tethers the polypeptide to the pentaglycine cell wall cross-bridge. During catalysis, the active site cysteine of sortase and the cleaved substrate form an acyl intermediate, which is then resolved by the amino group of pentaglycine cross-bridges. We report here the crystal structures of SrtBDeltaN30 in complex with two active site inhibitors, MTSET and E64, and with the cell wall substrate analog tripleglycine. These structures reveal, for the first time, the active site disposition and the unique Cys-Arg catalytic machinery of the cysteine transpeptidase, and they also provide useful information for the future design of anti-infective agents against sortases.
 ==About this Structure==
-QWZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with NI, SO4 and ETM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QWZ OCA].
+QWZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=NI:'>NI</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=ETM:'>ETM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWZ OCA].
 ==Reference==
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 [[Category: Single protein]]
 [[Category: Staphylococcus aureus]]
-[[Category: Mazmanian, S.K.]]
+[[Category: Mazmanian, S K.]]
-[[Category: Narayana, S.V.]]
+[[Category: Narayana, S V.]]
 [[Category: Schneewind, O.]]
 [[Category: Zong, Y.]]
@@ Line 23: / Line 23: @@
 [[Category: transpeptidase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:30:07 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:44:37 2008''