1qr4: Difference between revisions

Revision as of 15:42, 21 February 2008

TWO FIBRONECTIN TYPE-III DOMAIN SEGMENT FROM CHICKEN TENASCIN

OverviewOverview

A fragment of chicken tenascin consisting of fibronectin type-III domains 5 and 6 has been expressed in Escherichia coli. After modifying a previously reported purification protocol, an electrophoretically homogeneous recombinant protein was obtained from which various crystal forms could be grown under identical conditions. Only one form was suitable for structure determination. These crystals belong to space group P21, with unit-cell parameters a = 45.2, b = 57.9, c = 72.2 A, beta = 91.4 degrees, and diffract to at least 2.6 A resolution using synchrotron radiation. From density measurements of the crystals, it was found that there are two molecules in the asymmetric unit. Diffraction data of native, two platinum-derivative and one palladium-derivative crystals were collected.

About this StructureAbout this Structure

1QR4 is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

ReferenceReference

Purification, crystallization and preliminary crystallographic studies of a two fibronectin type-III domain segment from chicken tenascin encompassing the heparin- and contactin-binding regions., Bisig D, Weber P, Vaughan L, Winterhalter KH, Piontek K, Acta Crystallogr D Biol Crystallogr. 1999 May;55(Pt 5):1069-73. PMID:10216309

Page seeded by OCA on Thu Feb 21 14:42:46 2008

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OCA

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-[[Image:1qr4.gif|left|200px]]<br /><applet load="1qr4" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1qr4.gif|left|200px]]<br /><applet load="1qr4" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1qr4, resolution 2.55&Aring;" />
 '''TWO FIBRONECTIN TYPE-III DOMAIN SEGMENT FROM CHICKEN TENASCIN'''<br />
 ==Overview==
-A fragment of chicken tenascin consisting of fibronectin type-III domains, 5 and 6 has been expressed in Escherichia coli. After modifying a, previously reported purification protocol, an electrophoretically, homogeneous recombinant protein was obtained from which various crystal, forms could be grown under identical conditions. Only one form was, suitable for structure determination. These crystals belong to space group, P21, with unit-cell parameters a = 45.2, b = 57.9, c = 72.2 A, beta = 91.4, degrees, and diffract to at least 2.6 A resolution using synchrotron, radiation. From density measurements of the crystals, it was found that, there are two molecules in the asymmetric unit. Diffraction data of, native, two platinum-derivative and one palladium-derivative crystals were, collected.
+A fragment of chicken tenascin consisting of fibronectin type-III domains 5 and 6 has been expressed in Escherichia coli. After modifying a previously reported purification protocol, an electrophoretically homogeneous recombinant protein was obtained from which various crystal forms could be grown under identical conditions. Only one form was suitable for structure determination. These crystals belong to space group P21, with unit-cell parameters a = 45.2, b = 57.9, c = 72.2 A, beta = 91.4 degrees, and diffract to at least 2.6 A resolution using synchrotron radiation. From density measurements of the crystals, it was found that there are two molecules in the asymmetric unit. Diffraction data of native, two platinum-derivative and one palladium-derivative crystals were collected.
 ==About this Structure==
-QR4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QR4 OCA].
+QR4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QR4 OCA].
 ==Reference==
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 [[Category: Gallus gallus]]
 [[Category: Single protein]]
-[[Category: Bisig, D.A.]]
+[[Category: Bisig, D A.]]
 [[Category: Piontek, K.]]
 [[Category: adhesion]]
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 [[Category: tenascin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:54:38 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:42:46 2008''