1qfy: Difference between revisions

Revision as of 15:39, 21 February 2008

PEA FNR Y308S MUTANT IN COMPLEX WITH NADP+

OverviewOverview

The flavoenzyme ferredoxin-NADP+ reductase (FNR) catalyzes the production of NADPH during photosynthesis. Whereas the structures of FNRs from spinach leaf and a cyanobacterium as well as many of their homologs have been solved, none of these studies has yielded a productive geometry of the flavin-nicotinamide interaction. Here, we show that this failure occurs because nicotinamide binding to wild type FNR involves the energetically unfavorable displacement of the C-terminal Tyr side chain. We used mutants of this residue (Tyr 308) of pea FNR to obtain the structures of productive NADP+ and NADPH complexes. These structures reveal a unique NADP+ binding mode in which the nicotinamide ring is not parallel to the flavin isoalloxazine ring, but lies against it at an angle of approximately 30 degrees, with the C4 atom 3 A from the flavin N5 atom.

About this StructureAbout this Structure

1QFY is a Single protein structure of sequence from Pisum sativum with , and as ligands. Active as Ferredoxin--NADP(+) reductase, with EC number 1.18.1.2 Full crystallographic information is available from OCA.

ReferenceReference

A productive NADP+ binding mode of ferredoxin-NADP + reductase revealed by protein engineering and crystallographic studies., Deng Z, Aliverti A, Zanetti G, Arakaki AK, Ottado J, Orellano EG, Calcaterra NB, Ceccarelli EA, Carrillo N, Karplus PA, Nat Struct Biol. 1999 Sep;6(9):847-53. PMID:10467097

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OCA

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-[[Image:1qfy.gif|left|200px]]<br /><applet load="1qfy" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1qfy.gif|left|200px]]<br /><applet load="1qfy" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1qfy, resolution 1.8&Aring;" />
 '''PEA FNR Y308S MUTANT IN COMPLEX WITH NADP+'''<br />
 ==Overview==
-The flavoenzyme ferredoxin-NADP+ reductase (FNR) catalyzes the production, of NADPH during photosynthesis. Whereas the structures of FNRs from, spinach leaf and a cyanobacterium as well as many of their homologs have, been solved, none of these studies has yielded a productive geometry of, the flavin-nicotinamide interaction. Here, we show that this failure, occurs because nicotinamide binding to wild type FNR involves the, energetically unfavorable displacement of the C-terminal Tyr side chain., We used mutants of this residue (Tyr 308) of pea FNR to obtain the, structures of productive NADP+ and NADPH complexes. These structures, reveal a unique NADP+ binding mode in which the nicotinamide ring is not, parallel to the flavin isoalloxazine ring, but lies against it at an angle, of approximately 30 degrees, with the C4 atom 3 A from the flavin N5 atom.
+The flavoenzyme ferredoxin-NADP+ reductase (FNR) catalyzes the production of NADPH during photosynthesis. Whereas the structures of FNRs from spinach leaf and a cyanobacterium as well as many of their homologs have been solved, none of these studies has yielded a productive geometry of the flavin-nicotinamide interaction. Here, we show that this failure occurs because nicotinamide binding to wild type FNR involves the energetically unfavorable displacement of the C-terminal Tyr side chain. We used mutants of this residue (Tyr 308) of pea FNR to obtain the structures of productive NADP+ and NADPH complexes. These structures reveal a unique NADP+ binding mode in which the nicotinamide ring is not parallel to the flavin isoalloxazine ring, but lies against it at an angle of approximately 30 degrees, with the C4 atom 3 A from the flavin N5 atom.
 ==About this Structure==
-QFY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum] with SO4, FAD and NAP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QFY OCA].
+QFY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=NAP:'>NAP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QFY OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Single protein]]
 [[Category: Aliverti, A.]]
-[[Category: Arakaki, A.K.]]
+[[Category: Arakaki, A K.]]
-[[Category: Calcaterra, N.B.]]
+[[Category: Calcaterra, N B.]]
 [[Category: Carrillo, N.]]
-[[Category: Ceccarelli, E.A.]]
+[[Category: Ceccarelli, E A.]]
 [[Category: Deng, Z.]]
-[[Category: Karplus, P.A.]]
+[[Category: Karplus, P A.]]
-[[Category: Orellano, E.G.]]
+[[Category: Orellano, E G.]]
 [[Category: Ottado, J.]]
 [[Category: Zanetti, G.]]
@@ Line 32: / Line 32: @@
 [[Category: photosynthesis]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:39:18 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:39:05 2008''