1q72: Difference between revisions

Revision as of 15:36, 21 February 2008

Anti-Cocaine Antibody M82G2 Complexed with Cocaine

OverviewOverview

Antibodies against cocaine and other drugs of abuse are the basis for diagnostic tests for the presence of those drugs in human serum. The 1.7A resolution crystal structure of the anti-cocaine monoclonal antibody M82G2 in complex with cocaine is presented. This structure determination was undertaken to establish the stereochemical features in the antibody binding site that confer specificity for cocaine, and as part of an ongoing project to understand the rules that govern molecular recognition. The cocaine-binding site can be characterized topologically as a narrow groove on the protein surface. The antibody utilizes water-mediated hydrogen bonding, and cation-pi and stacking (pi-pi) interactions to provide specificity. Comparison with the previously published structure of the anti-cocaine antibody GNC92H2 shows that binding of a small ligand can be achieved in diverse ways, both in terms of a binding site structure/topology and protein-ligand interactions.

About this StructureAbout this Structure

1Q72 is a Protein complex structure of sequences from Mus musculus with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Diversity in hapten recognition: structural study of an anti-cocaine antibody M82G2., Pozharski E, Moulin A, Hewagama A, Shanafelt AB, Petsko GA, Ringe D, J Mol Biol. 2005 Jun 10;349(3):570-82. Epub 2005 Apr 21. PMID:15885702

Page seeded by OCA on Thu Feb 21 14:36:33 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1q72.gif|left|200px]]<br />
+[[Image:1q72.gif|left|200px]]<br /><applet load="1q72" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1q72" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1q72, resolution 1.70&Aring;" />
 '''Anti-Cocaine Antibody M82G2 Complexed with Cocaine'''<br />
 ==Overview==
-Antibodies against cocaine and other drugs of abuse are the basis for, diagnostic tests for the presence of those drugs in human serum. The 1.7A, resolution crystal structure of the anti-cocaine monoclonal antibody M82G2, in complex with cocaine is presented. This structure determination was, undertaken to establish the stereochemical features in the antibody, binding site that confer specificity for cocaine, and as part of an, ongoing project to understand the rules that govern molecular recognition., The cocaine-binding site can be characterized topologically as a narrow, groove on the protein surface. The antibody utilizes water-mediated, hydrogen bonding, and cation-pi and stacking (pi-pi) interactions to, provide specificity. Comparison with the previously published structure of, the anti-cocaine antibody GNC92H2 shows that binding of a small ligand can, be achieved in diverse ways, both in terms of a binding site, structure/topology and protein-ligand interactions.
+Antibodies against cocaine and other drugs of abuse are the basis for diagnostic tests for the presence of those drugs in human serum. The 1.7A resolution crystal structure of the anti-cocaine monoclonal antibody M82G2 in complex with cocaine is presented. This structure determination was undertaken to establish the stereochemical features in the antibody binding site that confer specificity for cocaine, and as part of an ongoing project to understand the rules that govern molecular recognition. The cocaine-binding site can be characterized topologically as a narrow groove on the protein surface. The antibody utilizes water-mediated hydrogen bonding, and cation-pi and stacking (pi-pi) interactions to provide specificity. Comparison with the previously published structure of the anti-cocaine antibody GNC92H2 shows that binding of a small ligand can be achieved in diverse ways, both in terms of a binding site structure/topology and protein-ligand interactions.
 ==About this Structure==
-Q72 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with COC as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q72 OCA].
+Q72 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=COC:'>COC</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q72 OCA].
 ==Reference==
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 [[Category: Hewagama, A.]]
 [[Category: Moulin, A.]]
-[[Category: Petsko, G.A.]]
+[[Category: Petsko, G A.]]
 [[Category: Pozharski, E.]]
 [[Category: Ringe, D.]]
-[[Category: Shanafelt, A.B.]]
+[[Category: Shanafelt, A B.]]
 [[Category: COC]]
 [[Category: anti-cocaine antibody]]
 [[Category: fab]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:39:58 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:36:33 2008''