1q0s: Difference between revisions

Revision as of 15:34, 21 February 2008

Binary Structure of T4DAM with AdoHcy

OverviewOverview

DNA-adenine methylation at certain GATC sites plays a pivotal role in bacterial and phage gene expression as well as bacterial virulence. We report here the crystal structures of the bacteriophage T4Dam DNA adenine methyltransferase (MTase) in a binary complex with the methyl-donor product S-adenosyl-L-homocysteine (AdoHcy) and in a ternary complex with a synthetic 12-bp DNA duplex and AdoHcy. T4Dam contains two domains: a seven-stranded catalytic domain that harbors the binding site for AdoHcy and a DNA binding domain consisting of a five-helix bundle and a beta-hairpin that is conserved in the family of GATC-related MTase orthologs. Unexpectedly, the sequence-specific T4Dam bound to DNA in a nonspecific mode that contained two Dam monomers per synthetic duplex, even though the DNA contains a single GATC site. The ternary structure provides a rare snapshot of an enzyme poised for linear diffusion along the DNA.

About this StructureAbout this Structure

1Q0S is a Single protein structure of sequence from Bacteriophage t4 with as ligand. Active as Site-specific DNA-methyltransferase (adenine-specific), with EC number 2.1.1.72 Full crystallographic information is available from OCA.

ReferenceReference

Structure of the bacteriophage T4 DNA adenine methyltransferase., Yang Z, Horton JR, Zhou L, Zhang XJ, Dong A, Zhang X, Schlagman SL, Kossykh V, Hattman S, Cheng X, Nat Struct Biol. 2003 Oct;10(10):849-55. Epub 2003 Aug 24. PMID:12937411

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OCA

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-[[Image:1q0s.jpg|left|200px]]<br /><applet load="1q0s" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1q0s.jpg|left|200px]]<br /><applet load="1q0s" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1q0s, resolution 2.3&Aring;" />
 '''Binary Structure of T4DAM with AdoHcy'''<br />
 ==Overview==
-DNA-adenine methylation at certain GATC sites plays a pivotal role in, bacterial and phage gene expression as well as bacterial virulence. We, report here the crystal structures of the bacteriophage T4Dam DNA adenine, methyltransferase (MTase) in a binary complex with the methyl-donor, product S-adenosyl-L-homocysteine (AdoHcy) and in a ternary complex with a, synthetic 12-bp DNA duplex and AdoHcy. T4Dam contains two domains: a, seven-stranded catalytic domain that harbors the binding site for AdoHcy, and a DNA binding domain consisting of a five-helix bundle and a, beta-hairpin that is conserved in the family of GATC-related MTase, orthologs. Unexpectedly, the sequence-specific T4Dam bound to DNA in a, nonspecific mode that contained two Dam monomers per synthetic duplex, even though the DNA contains a single GATC site. The ternary structure, provides a rare snapshot of an enzyme poised for linear diffusion along, the DNA.
+DNA-adenine methylation at certain GATC sites plays a pivotal role in bacterial and phage gene expression as well as bacterial virulence. We report here the crystal structures of the bacteriophage T4Dam DNA adenine methyltransferase (MTase) in a binary complex with the methyl-donor product S-adenosyl-L-homocysteine (AdoHcy) and in a ternary complex with a synthetic 12-bp DNA duplex and AdoHcy. T4Dam contains two domains: a seven-stranded catalytic domain that harbors the binding site for AdoHcy and a DNA binding domain consisting of a five-helix bundle and a beta-hairpin that is conserved in the family of GATC-related MTase orthologs. Unexpectedly, the sequence-specific T4Dam bound to DNA in a nonspecific mode that contained two Dam monomers per synthetic duplex, even though the DNA contains a single GATC site. The ternary structure provides a rare snapshot of an enzyme poised for linear diffusion along the DNA.
 ==About this Structure==
-Q0S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with SAH as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q0S OCA].
+Q0S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with <scene name='pdbligand=SAH:'>SAH</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q0S OCA].
 ==Reference==
@@ Line 17: / Line 17: @@
 [[Category: Dong, A.]]
 [[Category: Hattman, S.]]
-[[Category: Horton, J.R.]]
+[[Category: Horton, J R.]]
 [[Category: Kossykh, V.]]
-[[Category: Schlagman, S.L.]]
+[[Category: Schlagman, S L.]]
 [[Category: Yang, Z.]]
 [[Category: Zhang, X.]]
-[[Category: Zhang, X.J.]]
+[[Category: Zhang, X J.]]
 [[Category: Zhou, L.]]
 [[Category: SAH]]
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 [[Category: t4dam]]
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