1pvx: Difference between revisions

Revision as of 15:33, 21 February 2008

DO-1,4-BETA-XYLANASE, ROOM TEMPERATURE, PH 4.5

OverviewOverview

A highly thermostable xylanase isolated from the thermophilic fungus Paecilomyces varioti has been crystallized by the vapour diffusion method. The isolation of this enzyme by crystallization directly from the culture filtrate projects this fungus as an important source for large-scale production of pure xylanase. The crystals belong to orthorhombic space group P2(1)2(1)2(1) with the unit cell dimensions a = 38.48 A, b = 53.87 A and c = 90.23 A. Four molecules occupy a volume of 187,039.4 A3 along with 34% of solvent. The data collected with an area detector to the resolution of 2.7 A were used to calculate the unit cell parameters and Matthews' constant. The optical behaviour of the crystal was studied at different temperatures to understand its thermal stability.

About this StructureAbout this Structure

1PVX is a Single protein structure of sequence from Paecilomyces variotii. Active as Endo-1,4-beta-xylanase, with EC number 3.2.1.8 Full crystallographic information is available from OCA.

ReferenceReference

Crystallization and preliminary X-ray crystallographic studies of thermostable xylanase crystals isolated from Paecilomyces varioti., Eswaramoorthy S, Vithayathil PJ, Viswamitra MA, J Mol Biol. 1994 Nov 4;243(4):806-8. PMID:7966300

Page seeded by OCA on Thu Feb 21 14:33:07 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1pvx.jpg|left|200px]]<br /><applet load="1pvx" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1pvx.jpg|left|200px]]<br /><applet load="1pvx" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1pvx, resolution 1.59&Aring;" />
 '''DO-1,4-BETA-XYLANASE, ROOM TEMPERATURE, PH 4.5'''<br />
 ==Overview==
-A highly thermostable xylanase isolated from the thermophilic fungus, Paecilomyces varioti has been crystallized by the vapour diffusion method., The isolation of this enzyme by crystallization directly from the culture, filtrate projects this fungus as an important source for large-scale, production of pure xylanase. The crystals belong to orthorhombic space, group P2(1)2(1)2(1) with the unit cell dimensions a = 38.48 A, b = 53.87 A, and c = 90.23 A. Four molecules occupy a volume of 187,039.4 A3 along with, 34% of solvent. The data collected with an area detector to the resolution, of 2.7 A were used to calculate the unit cell parameters and Matthews', constant. The optical behaviour of the crystal was studied at different, temperatures to understand its thermal stability.
+A highly thermostable xylanase isolated from the thermophilic fungus Paecilomyces varioti has been crystallized by the vapour diffusion method. The isolation of this enzyme by crystallization directly from the culture filtrate projects this fungus as an important source for large-scale production of pure xylanase. The crystals belong to orthorhombic space group P2(1)2(1)2(1) with the unit cell dimensions a = 38.48 A, b = 53.87 A and c = 90.23 A. Four molecules occupy a volume of 187,039.4 A3 along with 34% of solvent. The data collected with an area detector to the resolution of 2.7 A were used to calculate the unit cell parameters and Matthews' constant. The optical behaviour of the crystal was studied at different temperatures to understand its thermal stability.
 ==About this Structure==
-PVX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paecilomyces_variotii Paecilomyces variotii]. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PVX OCA].
+PVX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paecilomyces_variotii Paecilomyces variotii]. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVX OCA].
 ==Reference==
@@ Line 16: / Line 16: @@
 [[Category: Eswaramoorthy, S.]]
 [[Category: Rajeshkumar, P.]]
-[[Category: Viswamitra, M.A.]]
+[[Category: Viswamitra, M A.]]
-[[Category: Vithayathil, P.J.]]
+[[Category: Vithayathil, P J.]]
 [[Category: family-11 of glycosyl hydrolases]]
 [[Category: thermophilic]]
 [[Category: xylanase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:10:14 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:07 2008''