1psk: Difference between revisions

Revision as of 15:32, 21 February 2008

THE CRYSTAL STRUCTURE OF AN FAB FRAGMENT THAT BINDS TO THE MELANOMA-ASSOCIATED GD2 GANGLIOSIDE

OverviewOverview

The GD2 ganglioside is a cell-surface component that appears on the surface of metastatic melanoma cells and is a marker for the progression of the disease. The ME36.1 monoclonal antibody binds to the GD2 ganglioside and has shown potential as a therapeutic antibody. ME36.1 is a possible alternative therapy to radiation, which is often ineffective in late-stage melanoma. The crystal structure of the Fab fragment of ME36.1 has been determined using molecular replacement and refined to an R factor of 20.4% at 2.8 A resolution. The model has good geometry with root-mean-square deviations of 0.008 A from ideal bond lengths and 1.7 degrees from ideal bond angles. The crystal structure of the ME36.1 Fab shows that its complementarity determining region forms a groove-shaped binding site rather than the pocket-type observed in other sugar binding Fabs. Molecular modeling has placed a four-residue sugar, representative of GD2, in the antigen binding site. The GD2 sugar moiety is stabilized by a network of hydrogen bonds that define the specificity of ME36.1 toward its antigen.

About this StructureAbout this Structure

1PSK is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of a Fab fragment to the melanoma-associated GD2 ganglioside., Pichla SL, Murali R, Burnett RM, J Struct Biol. 1997 Jun;119(1):6-16. PMID:9216084

Page seeded by OCA on Thu Feb 21 14:32:06 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1psk.gif|left|200px]]<br />
+[[Image:1psk.gif|left|200px]]<br /><applet load="1psk" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1psk" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1psk, resolution 2.8&Aring;" />
 '''THE CRYSTAL STRUCTURE OF AN FAB FRAGMENT THAT BINDS TO THE MELANOMA-ASSOCIATED GD2 GANGLIOSIDE'''<br />
 ==Overview==
-The GD2 ganglioside is a cell-surface component that appears on the, surface of metastatic melanoma cells and is a marker for the progression, of the disease. The ME36.1 monoclonal antibody binds to the GD2, ganglioside and has shown potential as a therapeutic antibody. ME36.1 is a, possible alternative therapy to radiation, which is often ineffective in, late-stage melanoma. The crystal structure of the Fab fragment of ME36.1, has been determined using molecular replacement and refined to an R factor, of 20.4% at 2.8 A resolution. The model has good geometry with, root-mean-square deviations of 0.008 A from ideal bond lengths and 1.7, degrees from ideal bond angles. The crystal structure of the ME36.1 Fab, shows that its complementarity determining region forms a groove-shaped, binding site rather than the pocket-type observed in other sugar binding, Fabs. Molecular modeling has placed a four-residue sugar, representative, of GD2, in the antigen binding site. The GD2 sugar moiety is stabilized by, a network of hydrogen bonds that define the specificity of ME36.1 toward, its antigen.
+The GD2 ganglioside is a cell-surface component that appears on the surface of metastatic melanoma cells and is a marker for the progression of the disease. The ME36.1 monoclonal antibody binds to the GD2 ganglioside and has shown potential as a therapeutic antibody. ME36.1 is a possible alternative therapy to radiation, which is often ineffective in late-stage melanoma. The crystal structure of the Fab fragment of ME36.1 has been determined using molecular replacement and refined to an R factor of 20.4% at 2.8 A resolution. The model has good geometry with root-mean-square deviations of 0.008 A from ideal bond lengths and 1.7 degrees from ideal bond angles. The crystal structure of the ME36.1 Fab shows that its complementarity determining region forms a groove-shaped binding site rather than the pocket-type observed in other sugar binding Fabs. Molecular modeling has placed a four-residue sugar, representative of GD2, in the antigen binding site. The GD2 sugar moiety is stabilized by a network of hydrogen bonds that define the specificity of ME36.1 toward its antigen.
 ==About this Structure==
-PSK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PSK OCA].
+PSK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PSK OCA].
 ==Reference==
@@ Line 14: / Line 13: @@
 [[Category: Mus musculus]]
 [[Category: Protein complex]]
-[[Category: Burnett, R.M.]]
+[[Category: Burnett, R M.]]
 [[Category: Murali, R.]]
-[[Category: Pichla, S.L.]]
+[[Category: Pichla, S L.]]
 [[Category: carbohydrate]]
 [[Category: fab]]
@@ Line 23: / Line 22: @@
 [[Category: melanoma]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:39:47 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:32:06 2008''