1pdn: Difference between revisions

Revision as of 15:27, 21 February 2008

CRYSTAL STRUCTURE OF A PAIRED DOMAIN-DNA COMPLEX AT 2.5 ANGSTROMS RESOLUTION REVEALS STRUCTURAL BASIS FOR PAX DEVELOPMENTAL MUTATIONS

OverviewOverview

The 2.5 A resolution structure of a cocrystal containing the paired domain from the Drosophila paired (prd) protein and a 15 bp site shows structurally independent N-terminal and C-terminal subdomains. Each of these domains contains a helical region resembling the homeodomain and the Hin recombinase. The N-terminal domain makes extensive DNA contacts, using a novel beta turn motif that binds in the minor groove and a helix-turn-helix unit with a docking arrangement surprisingly similar to that of the lambda repressor. The C-terminal domain is not essential for prd binding and does not contact the optimized site. All known developmental missense mutations in the paired box of mammalian Pax genes map to the N-terminal subdomain, and most of them are found at the protein-DNA interface.

About this StructureAbout this Structure

1PDN is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of a paired domain-DNA complex at 2.5 A resolution reveals structural basis for Pax developmental mutations., Xu W, Rould MA, Jun S, Desplan C, Pabo CO, Cell. 1995 Feb 24;80(4):639-50. PMID:7867071

Page seeded by OCA on Thu Feb 21 14:27:43 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1pdn.jpg|left|200px]]<br /><applet load="1pdn" size="450" color="white" frame="true" align="right" spinBox="true"
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 caption="1pdn, resolution 2.500&Aring;" />
 '''CRYSTAL STRUCTURE OF A PAIRED DOMAIN-DNA COMPLEX AT 2.5 ANGSTROMS RESOLUTION REVEALS STRUCTURAL BASIS FOR PAX DEVELOPMENTAL MUTATIONS'''<br />
 ==Overview==
-The 2.5 A resolution structure of a cocrystal containing the paired domain, from the Drosophila paired (prd) protein and a 15 bp site shows, structurally independent N-terminal and C-terminal subdomains. Each of, these domains contains a helical region resembling the homeodomain and the, Hin recombinase. The N-terminal domain makes extensive DNA contacts, using, a novel beta turn motif that binds in the minor groove and a, helix-turn-helix unit with a docking arrangement surprisingly similar to, that of the lambda repressor. The C-terminal domain is not essential for, prd binding and does not contact the optimized site. All known, developmental missense mutations in the paired box of mammalian Pax genes, map to the N-terminal subdomain, and most of them are found at the, protein-DNA interface.
+The 2.5 A resolution structure of a cocrystal containing the paired domain from the Drosophila paired (prd) protein and a 15 bp site shows structurally independent N-terminal and C-terminal subdomains. Each of these domains contains a helical region resembling the homeodomain and the Hin recombinase. The N-terminal domain makes extensive DNA contacts, using a novel beta turn motif that binds in the minor groove and a helix-turn-helix unit with a docking arrangement surprisingly similar to that of the lambda repressor. The C-terminal domain is not essential for prd binding and does not contact the optimized site. All known developmental missense mutations in the paired box of mammalian Pax genes map to the N-terminal subdomain, and most of them are found at the protein-DNA interface.
 ==About this Structure==
-PDN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PDN OCA].
+PDN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PDN OCA].
 ==Reference==
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 [[Category: Desplan, C.]]
 [[Category: Jun, S.]]
-[[Category: Pabo, C.O.]]
+[[Category: Pabo, C O.]]
-[[Category: Rould, M.A.]]
+[[Category: Rould, M A.]]
 [[Category: Xu, W.]]
 [[Category: dna-binding protein]]
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 [[Category: protein-dna complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:43:05 2007''
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