1ot2: Difference between revisions

Revision as of 15:21, 21 February 2008

Bacillus circulans strain 251 Cyclodextrin glycosyl transferase mutant D135N

OverviewOverview

The alpha-amylase family is a large group of starch processing enzymes [Svensson, B. (1994) Plant Mol. Biol. 25, 141-157]. It is characterized by four short sequence motifs that contain the seven fully conserved amino acid residues in this family: two catalytic carboxylic acid residues and four substrate binding residues. The seventh conserved residue (Asp135) has no direct interactions with either substrates or products, but it is hydrogen-bonded to Arg227, which does bind the substrate in the catalytic site. Using cyclodextrin glycosyltransferase as an example, this paper provides for the first time definite biochemical and structural evidence that Asp135 is required for the proper conformation of several catalytic site residues and therefore for activity.

About this StructureAbout this Structure

1OT2 is a Single protein structure of sequence from Bacillus circulans with , , , , and as ligands. Active as Cyclomaltodextrin glucanotransferase, with EC number 2.4.1.19 Full crystallographic information is available from OCA.

ReferenceReference

The fully conserved Asp residue in conserved sequence region I of the alpha-amylase family is crucial for the catalytic site architecture and activity., Leemhuis H, Rozeboom HJ, Dijkstra BW, Dijkhuizen L, FEBS Lett. 2003 Apr 24;541(1-3):47-51. PMID:12706817

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OCA

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-[[Image:1ot2.jpg|left|200px]]<br /><applet load="1ot2" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ot2.jpg|left|200px]]<br /><applet load="1ot2" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ot2, resolution 2.10&Aring;" />
 '''Bacillus circulans strain 251 Cyclodextrin glycosyl transferase mutant D135N'''<br />
 ==Overview==
-The alpha-amylase family is a large group of starch processing enzymes, [Svensson, B. (1994) Plant Mol. Biol. 25, 141-157]. It is characterized by, four short sequence motifs that contain the seven fully conserved amino, acid residues in this family: two catalytic carboxylic acid residues and, four substrate binding residues. The seventh conserved residue (Asp135), has no direct interactions with either substrates or products, but it is, hydrogen-bonded to Arg227, which does bind the substrate in the catalytic, site. Using cyclodextrin glycosyltransferase as an example, this paper, provides for the first time definite biochemical and structural evidence, that Asp135 is required for the proper conformation of several catalytic, site residues and therefore for activity.
+The alpha-amylase family is a large group of starch processing enzymes [Svensson, B. (1994) Plant Mol. Biol. 25, 141-157]. It is characterized by four short sequence motifs that contain the seven fully conserved amino acid residues in this family: two catalytic carboxylic acid residues and four substrate binding residues. The seventh conserved residue (Asp135) has no direct interactions with either substrates or products, but it is hydrogen-bonded to Arg227, which does bind the substrate in the catalytic site. Using cyclodextrin glycosyltransferase as an example, this paper provides for the first time definite biochemical and structural evidence that Asp135 is required for the proper conformation of several catalytic site residues and therefore for activity.
 ==About this Structure==
-OT2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with MAL, GLC, CA, EPE, MPD and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OT2 OCA].
+OT2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with <scene name='pdbligand=MAL:'>MAL</scene>, <scene name='pdbligand=GLC:'>GLC</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=EPE:'>EPE</scene>, <scene name='pdbligand=MPD:'>MPD</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OT2 OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Cyclomaltodextrin glucanotransferase]]
 [[Category: Single protein]]
-[[Category: Dijkstra, B.W.]]
+[[Category: Dijkstra, B W.]]
-[[Category: Rozeboom, H.J.]]
+[[Category: Rozeboom, H J.]]
 [[Category: ACY]]
 [[Category: CA]]
@@ Line 25: / Line 25: @@
 [[Category: glycosyl transferase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:10:14 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:21:14 2008''