1nw2: Difference between revisions
New page: left|200px<br /><applet load="1nw2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nw2, resolution 1.90Å" /> '''The crystal structur... |
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[[Image:1nw2.jpg|left|200px]]<br /><applet load="1nw2" size=" | [[Image:1nw2.jpg|left|200px]]<br /><applet load="1nw2" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1nw2, resolution 1.90Å" /> | caption="1nw2, resolution 1.90Å" /> | ||
'''The crystal structure of the mutant R82E of Thioredoxin from Alicyclobacillus acidocaldarius'''<br /> | '''The crystal structure of the mutant R82E of Thioredoxin from Alicyclobacillus acidocaldarius'''<br /> | ||
==Overview== | ==Overview== | ||
We report a crystallographic and computational analysis of two mutant | We report a crystallographic and computational analysis of two mutant forms of the Alicyclobacillus acidocaldarius thioredoxin (BacTrx) done in order to evaluate the contribution of two specific amino acids to the thermostability of BacTrx. Our results suggest that the thermostability of BacTrx may be modulated by mutations affecting the overall electrostatic energy of the protein. | ||
==About this Structure== | ==About this Structure== | ||
1NW2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius Alicyclobacillus acidocaldarius] with CAC, ZN and ACT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] Full crystallographic information is available from [http:// | 1NW2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius Alicyclobacillus acidocaldarius] with <scene name='pdbligand=CAC:'>CAC</scene>, <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=ACT:'>ACT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NW2 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Pedone, E.]] | [[Category: Pedone, E.]] | ||
[[Category: Saviano, M.]] | [[Category: Saviano, M.]] | ||
[[Category: Simone, G | [[Category: Simone, G De.]] | ||
[[Category: ACT]] | [[Category: ACT]] | ||
[[Category: CAC]] | [[Category: CAC]] | ||
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[[Category: thioredoxin]] | [[Category: thioredoxin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:10:41 2008'' | ||
Revision as of 15:10, 21 February 2008
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The crystal structure of the mutant R82E of Thioredoxin from Alicyclobacillus acidocaldarius
OverviewOverview
We report a crystallographic and computational analysis of two mutant forms of the Alicyclobacillus acidocaldarius thioredoxin (BacTrx) done in order to evaluate the contribution of two specific amino acids to the thermostability of BacTrx. Our results suggest that the thermostability of BacTrx may be modulated by mutations affecting the overall electrostatic energy of the protein.
About this StructureAbout this Structure
1NW2 is a Single protein structure of sequence from Alicyclobacillus acidocaldarius with , and as ligands. Active as Thioredoxin-disulfide reductase, with EC number 1.8.1.9 Full crystallographic information is available from OCA.
ReferenceReference
An integrated structural and computational study of the thermostability of two thioredoxin mutants from Alicyclobacillus acidocaldarius., Bartolucci S, De Simone G, Galdiero S, Improta R, Menchise V, Pedone C, Pedone E, Saviano M, J Bacteriol. 2003 Jul;185(14):4285-9. PMID:12837806
Page seeded by OCA on Thu Feb 21 14:10:41 2008