1nu6: Difference between revisions

Revision as of 15:10, 21 February 2008

Crystal structure of human Dipeptidyl Peptidase IV (DPP-IV)

OverviewOverview

Inhibition of dipeptidyl peptidase IV (DPP-IV), the main glucagon-like peptide 1 (GLP1)-degrading enzyme, has been proposed for the treatment of type II diabetes. We expressed and purified the ectodomain of human DPP-IV in Pichia pastoris and determined the X-ray structure at 2.1 A resolution. The enzyme consists of two domains, the catalytic domain, with an alpha/beta hydrolase fold, and a beta propeller domain with an 8-fold repeat of a four-strand beta sheet motif. The beta propeller domain contributes two important functions to the molecule that have not been reported for such structures, an extra beta sheet motif that forms part of the dimerization interface and an additional short helix with a double Glu sequence motif. The Glu motif provides recognition and a binding site for the N terminus of the substrates, as revealed by the complex structure with diprotin A, a substrate with low turnover that is trapped in the tetrahedral intermediate of the reaction in the crystal.

About this StructureAbout this Structure

1NU6 is a Single protein structure of sequence from Homo sapiens with , and as ligands. Active as Dipeptidyl-peptidase IV, with EC number 3.4.14.5 Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of proline-specific exopeptidase activity as observed in human dipeptidyl peptidase-IV., Thoma R, Loffler B, Stihle M, Huber W, Ruf A, Hennig M, Structure. 2003 Aug;11(8):947-59. PMID:12906826

Page seeded by OCA on Thu Feb 21 14:10:01 2008

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OCA

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-[[Image:1nu6.gif|left|200px]]<br />
+[[Image:1nu6.gif|left|200px]]<br /><applet load="1nu6" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1nu6" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1nu6, resolution 2.10&Aring;" />
 '''Crystal structure of human Dipeptidyl Peptidase IV (DPP-IV)'''<br />
 ==Overview==
-Inhibition of dipeptidyl peptidase IV (DPP-IV), the main glucagon-like, peptide 1 (GLP1)-degrading enzyme, has been proposed for the treatment of, type II diabetes. We expressed and purified the ectodomain of human DPP-IV, in Pichia pastoris and determined the X-ray structure at 2.1 A resolution., The enzyme consists of two domains, the catalytic domain, with an, alpha/beta hydrolase fold, and a beta propeller domain with an 8-fold, repeat of a four-strand beta sheet motif. The beta propeller domain, contributes two important functions to the molecule that have not been, reported for such structures, an extra beta sheet motif that forms part of, the dimerization interface and an additional short helix with a double Glu, sequence motif. The Glu motif provides recognition and a binding site for, the N terminus of the substrates, as revealed by the complex structure, with diprotin A, a substrate with low turnover that is trapped in the, tetrahedral intermediate of the reaction in the crystal.
+Inhibition of dipeptidyl peptidase IV (DPP-IV), the main glucagon-like peptide 1 (GLP1)-degrading enzyme, has been proposed for the treatment of type II diabetes. We expressed and purified the ectodomain of human DPP-IV in Pichia pastoris and determined the X-ray structure at 2.1 A resolution. The enzyme consists of two domains, the catalytic domain, with an alpha/beta hydrolase fold, and a beta propeller domain with an 8-fold repeat of a four-strand beta sheet motif. The beta propeller domain contributes two important functions to the molecule that have not been reported for such structures, an extra beta sheet motif that forms part of the dimerization interface and an additional short helix with a double Glu sequence motif. The Glu motif provides recognition and a binding site for the N terminus of the substrates, as revealed by the complex structure with diprotin A, a substrate with low turnover that is trapped in the tetrahedral intermediate of the reaction in the crystal.
 ==About this Structure==
-NU6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAG, NDG and HG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Dipeptidyl-peptidase_IV Dipeptidyl-peptidase IV], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.14.5 3.4.14.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NU6 OCA].
+NU6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=NDG:'>NDG</scene> and <scene name='pdbligand=HG:'>HG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Dipeptidyl-peptidase_IV Dipeptidyl-peptidase IV], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.14.5 3.4.14.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU6 OCA].
 ==Reference==
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 [[Category: exopeptidase]]
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