1njf: Difference between revisions

Revision as of 15:06, 21 February 2008

Nucleotide bound form of an isolated E. coli clamp loader gamma subunit

OverviewOverview

Sliding clamps are loaded onto DNA by ATP-driven clamp loader complexes. The structure of the E. coli clamp loader in a nucleotide-free state has been determined previously. We now report crystal structures of a truncated form of the isolated gamma-ATPase subunit, gamma(1-243), of the E. coli clamp loader, in nucleotide-free and bound forms. The gamma subunit adopts a defined conformation when empty, in which the nucleotide binding site is blocked. The binding of either ATPgammaS or ADP, which are shown to bind with equal affinity to gamma(1-243), induces a change in the relative orientation of the two domains such that nucleotides can be accommodated. This change would break one of the gamma:gamma interfaces seen in the empty clamp loader complex, and may represent one step in the activation process.

About this StructureAbout this Structure

1NJF is a Single protein structure of sequence from Escherichia coli with , and as ligands. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.

ReferenceReference

Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit., Podobnik M, Weitze TF, O'Donnell M, Kuriyan J, Structure. 2003 Mar;11(3):253-63. PMID:12623013

Page seeded by OCA on Thu Feb 21 14:06:50 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1njf.gif|left|200px]]<br /><applet load="1njf" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1njf.gif|left|200px]]<br /><applet load="1njf" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1njf, resolution 2.30&Aring;" />
 '''Nucleotide bound form of an isolated E. coli clamp loader gamma subunit'''<br />
 ==Overview==
-Sliding clamps are loaded onto DNA by ATP-driven clamp loader complexes., The structure of the E. coli clamp loader in a nucleotide-free state has, been determined previously. We now report crystal structures of a, truncated form of the isolated gamma-ATPase subunit, gamma(1-243), of the, E. coli clamp loader, in nucleotide-free and bound forms. The gamma, subunit adopts a defined conformation when empty, in which the nucleotide, binding site is blocked. The binding of either ATPgammaS or ADP, which are, shown to bind with equal affinity to gamma(1-243), induces a change in the, relative orientation of the two domains such that nucleotides can be, accommodated. This change would break one of the gamma:gamma interfaces, seen in the empty clamp loader complex, and may represent one step in the, activation process.
+Sliding clamps are loaded onto DNA by ATP-driven clamp loader complexes. The structure of the E. coli clamp loader in a nucleotide-free state has been determined previously. We now report crystal structures of a truncated form of the isolated gamma-ATPase subunit, gamma(1-243), of the E. coli clamp loader, in nucleotide-free and bound forms. The gamma subunit adopts a defined conformation when empty, in which the nucleotide binding site is blocked. The binding of either ATPgammaS or ADP, which are shown to bind with equal affinity to gamma(1-243), induces a change in the relative orientation of the two domains such that nucleotides can be accommodated. This change would break one of the gamma:gamma interfaces seen in the empty clamp loader complex, and may represent one step in the activation process.
 ==About this Structure==
-NJF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN, ATG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NJF OCA].
+NJF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=ATG:'>ATG</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJF OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Donnell, M.O.]]
+[[Category: Donnell, M O.]]
 [[Category: Kuriyan, J.]]
 [[Category: Podobnik, M.]]
-[[Category: Weitze, T.F.]]
+[[Category: Weitze, T F.]]
 [[Category: ADP]]
 [[Category: ATG]]
@@ Line 27: / Line 27: @@
 [[Category: sensor 2]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:18:58 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:50 2008''