1ne3: Difference between revisions
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'''Solution structure of ribosomal protein S28E from Methanobacterium Thermoautotrophicum. Ontario Centre for Structural Proteomics target MTH0256_1_68; Northeast Structural Genomics Target TT744'''<br /> | '''Solution structure of ribosomal protein S28E from Methanobacterium Thermoautotrophicum. Ontario Centre for Structural Proteomics target MTH0256_1_68; Northeast Structural Genomics Target TT744'''<br /> | ||
==Overview== | ==Overview== | ||
The ribosomal protein S28E from the archaeon Methanobacterium | The ribosomal protein S28E from the archaeon Methanobacterium thermoautotrophicum is a component of the 30S ribosomal subunit. Sequence homologs of S28E are found only in archaea and eukaryotes. Here we report the three-dimensional solution structure of S28E by NMR spectroscopy. S28E contains a globular region and a long C-terminal tail protruding from the core. The globular region consists of four antiparallel beta-strands that are arranged in a Greek-key topology. Unique features of S28E include an extended loop L2-3 that folds back onto the protein and a 12-residue charged C-terminal tail with no regular secondary structure and greater flexibility relative to the rest of the protein. The structural and surface resemblance to OB-fold family of proteins and the presence of highly conserved basic residues suggest that S28E may bind to RNA. A broad positively charged surface extending over one side of the beta-barrel and into the flexible C terminus may present a putative binding site for RNA. | ||
==About this Structure== | ==About this Structure== | ||
1NE3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus Methanothermococcus thermolithotrophicus]. Full crystallographic information is available from [http:// | 1NE3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus Methanothermococcus thermolithotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NE3 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Methanothermococcus thermolithotrophicus]] | [[Category: Methanothermococcus thermolithotrophicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Arrowsmith, C | [[Category: Arrowsmith, C H.]] | ||
[[Category: Cort, J | [[Category: Cort, J R.]] | ||
[[Category: Edwards, A.]] | [[Category: Edwards, A.]] | ||
[[Category: Kennedy, M.]] | [[Category: Kennedy, M.]] | ||
[[Category: NESG, Northeast | [[Category: NESG, Northeast Structural Genomics Consortium.]] | ||
[[Category: Pineda-Lucena, A.]] | [[Category: Pineda-Lucena, A.]] | ||
[[Category: Ramelot, T | [[Category: Ramelot, T A.]] | ||
[[Category: Wu, B.]] | [[Category: Wu, B.]] | ||
[[Category: Yee, A.]] | [[Category: Yee, A.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:05 2008'' | ||
Revision as of 15:05, 21 February 2008
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Solution structure of ribosomal protein S28E from Methanobacterium Thermoautotrophicum. Ontario Centre for Structural Proteomics target MTH0256_1_68; Northeast Structural Genomics Target TT744
OverviewOverview
The ribosomal protein S28E from the archaeon Methanobacterium thermoautotrophicum is a component of the 30S ribosomal subunit. Sequence homologs of S28E are found only in archaea and eukaryotes. Here we report the three-dimensional solution structure of S28E by NMR spectroscopy. S28E contains a globular region and a long C-terminal tail protruding from the core. The globular region consists of four antiparallel beta-strands that are arranged in a Greek-key topology. Unique features of S28E include an extended loop L2-3 that folds back onto the protein and a 12-residue charged C-terminal tail with no regular secondary structure and greater flexibility relative to the rest of the protein. The structural and surface resemblance to OB-fold family of proteins and the presence of highly conserved basic residues suggest that S28E may bind to RNA. A broad positively charged surface extending over one side of the beta-barrel and into the flexible C terminus may present a putative binding site for RNA.
About this StructureAbout this Structure
1NE3 is a Single protein structure of sequence from Methanothermococcus thermolithotrophicus. Full crystallographic information is available from OCA.
ReferenceReference
Solution structure of ribosomal protein S28E from Methanobacterium thermoautotrophicum., Wu B, Yee A, Pineda-Lucena A, Semesi A, Ramelot TA, Cort JR, Jung JW, Edwards A, Lee W, Kennedy M, Arrowsmith CH, Protein Sci. 2003 Dec;12(12):2831-7. PMID:14627743
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Methanothermococcus thermolithotrophicus
- Single protein
- Arrowsmith, C H.
- Cort, J R.
- Edwards, A.
- Kennedy, M.
- NESG, Northeast Structural Genomics Consortium.
- Pineda-Lucena, A.
- Ramelot, T A.
- Wu, B.
- Yee, A.
- Beta protein
- Nesg
- Northeast structural genomics consortium
- Ocsp
- Protein structure initiative
- Psi
- Structural genomics