1n93: Difference between revisions

Revision as of 15:03, 21 February 2008

Crystal Structure of the Borna Disease Virus Nucleoprotein

OverviewOverview

Borna disease virus (BDV) causes an infection of the central nervous system in a wide range of vertebrates, which can fatally progress to an immune-mediated disease, called Borna disease. BDV is a member of the Mononegavirales, which also includes the highly infectious measles and Ebola viruses. The viral nucleoproteins are central to transcription, replication, and packaging of the RNA genome. We present the X-ray structure of the BDV nucleoprotein determined at 1.76 A resolution. The structure reveals a novel fold, organized into two distinct domains, and an assembly into a planar homotetramer. Surface potential calculations strongly support an RNA binding model with the RNA wrapping around the outside of the tetramer, although a positively charged central channel in the tetramer could fit single-stranded RNA in an alternative binding mode. This first structure of an RNA virus nucleoprotein provides a paradigmatic model for RNA packaging and replication of single-stranded RNA viruses.

About this StructureAbout this Structure

1N93 is a Single protein structure of sequence from Borna disease virus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the borna disease virus nucleoprotein., Rudolph MG, Kraus I, Dickmanns A, Eickmann M, Garten W, Ficner R, Structure. 2003 Oct;11(10):1219-26. PMID:14527390

Page seeded by OCA on Thu Feb 21 14:03:32 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1n93.gif|left|200px]]<br /><applet load="1n93" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1n93.gif|left|200px]]<br /><applet load="1n93" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1n93, resolution 1.76&Aring;" />
 '''Crystal Structure of the Borna Disease Virus Nucleoprotein'''<br />
 ==Overview==
-Borna disease virus (BDV) causes an infection of the central nervous, system in a wide range of vertebrates, which can fatally progress to an, immune-mediated disease, called Borna disease. BDV is a member of the, Mononegavirales, which also includes the highly infectious measles and, Ebola viruses. The viral nucleoproteins are central to transcription, replication, and packaging of the RNA genome. We present the X-ray, structure of the BDV nucleoprotein determined at 1.76 A resolution. The, structure reveals a novel fold, organized into two distinct domains, and, an assembly into a planar homotetramer. Surface potential calculations, strongly support an RNA binding model with the RNA wrapping around the, outside of the tetramer, although a positively charged central channel in, the tetramer could fit single-stranded RNA in an alternative binding mode., This first structure of an RNA virus nucleoprotein provides a paradigmatic, model for RNA packaging and replication of single-stranded RNA viruses.
+Borna disease virus (BDV) causes an infection of the central nervous system in a wide range of vertebrates, which can fatally progress to an immune-mediated disease, called Borna disease. BDV is a member of the Mononegavirales, which also includes the highly infectious measles and Ebola viruses. The viral nucleoproteins are central to transcription, replication, and packaging of the RNA genome. We present the X-ray structure of the BDV nucleoprotein determined at 1.76 A resolution. The structure reveals a novel fold, organized into two distinct domains, and an assembly into a planar homotetramer. Surface potential calculations strongly support an RNA binding model with the RNA wrapping around the outside of the tetramer, although a positively charged central channel in the tetramer could fit single-stranded RNA in an alternative binding mode. This first structure of an RNA virus nucleoprotein provides a paradigmatic model for RNA packaging and replication of single-stranded RNA viruses.
 ==About this Structure==
-N93 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Borna_disease_virus Borna disease virus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N93 OCA].
+N93 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Borna_disease_virus Borna disease virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N93 OCA].
 ==Reference==
@@ Line 17: / Line 17: @@
 [[Category: Garten, W.]]
 [[Category: Kraus, I.]]
-[[Category: Rudolph, M.G.]]
+[[Category: Rudolph, M G.]]
 [[Category: rna virus nucleoprotein tetramer]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:03:59 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:32 2008''