1mm3: Difference between revisions
New page: left|200px<br /><applet load="1mm3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mm3" /> '''Solution structure of the 2nd PHD domain fro... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1mm3.gif|left|200px]]<br /><applet load="1mm3" size=" | [[Image:1mm3.gif|left|200px]]<br /><applet load="1mm3" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1mm3" /> | caption="1mm3" /> | ||
'''Solution structure of the 2nd PHD domain from Mi2b with C-terminal loop replaced by corresponding loop from WSTF'''<br /> | '''Solution structure of the 2nd PHD domain from Mi2b with C-terminal loop replaced by corresponding loop from WSTF'''<br /> | ||
==Overview== | ==Overview== | ||
The design of proteins with tailored functions remains a relatively | The design of proteins with tailored functions remains a relatively elusive goal. Small size, a well-defined structure, and the ability to maintain structural integrity despite multiple mutations are all desirable properties for such designer proteins. Many zinc binding domains fit this description. We determined the structure of a PHD finger from the transcriptional cofactor Mi2beta and investigated the suitability of this domain as a scaffold for presenting selected binding functions. The two flexible loops in the structure were mutated extensively by either substitution or expansion, without affecting the overall fold of the domain. A binding site for the corepressor CtBP2 was also grafted onto the domain, creating a new PHD domain that can specifically bind CtBP2 both in vitro and in the context of a eukaryotic cell nucleus. These results represent a step toward designing new regulatory proteins for modulating aberrant gene expression in vivo. | ||
==About this Structure== | ==About this Structure== | ||
1MM3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1MM3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM3 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Crossley, M.]] | [[Category: Crossley, M.]] | ||
[[Category: Gell, D | [[Category: Gell, D A.]] | ||
[[Category: Kwan, A | [[Category: Kwan, A H.Y.]] | ||
[[Category: Mackay, J | [[Category: Mackay, J P.]] | ||
[[Category: Matthews, J | [[Category: Matthews, J M.]] | ||
[[Category: Verger, A.]] | [[Category: Verger, A.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
| Line 24: | Line 24: | ||
[[Category: zinc finger]] | [[Category: zinc finger]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:56:35 2008'' | ||
