1mix: Difference between revisions
New page: left|200px<br /><applet load="1mix" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mix, resolution 1.75Å" /> '''Crystal structure of... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1mix.gif|left|200px]]<br /><applet load="1mix" size=" | [[Image:1mix.gif|left|200px]]<br /><applet load="1mix" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1mix, resolution 1.75Å" /> | caption="1mix, resolution 1.75Å" /> | ||
'''Crystal structure of a FERM domain of Talin'''<br /> | '''Crystal structure of a FERM domain of Talin'''<br /> | ||
==Overview== | ==Overview== | ||
The binding of cytoplasmic proteins, such as talin, to the cytoplasmic | The binding of cytoplasmic proteins, such as talin, to the cytoplasmic domains of integrin adhesion receptors mediates bidirectional signal transduction. Here we report the crystal structure of the principal integrin binding and activating fragment of talin, alone and in complex with fragments of the beta 3 integrin tail. The FERM (four point one, ezrin, radixin, and moesin) domain of talin engages integrins via a novel variant of the canonical phosphotyrosine binding (PTB) domain-NPxY ligand interaction that may be a prototype for FERM domain recognition of transmembrane receptors. In combination with NMR and mutational analysis, our studies reveal the critical interacting elements of both talin and the integrin beta 3 tail, providing structural paradigms for integrin linkage to the cell interior. | ||
==About this Structure== | ==About this Structure== | ||
1MIX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http:// | 1MIX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIX OCA]. | ||
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Calderwood, D | [[Category: Calderwood, D A.]] | ||
[[Category: Campbell, I | [[Category: Campbell, I D.]] | ||
[[Category: Critchley, D.]] | [[Category: Critchley, D.]] | ||
[[Category: Garcia-Alvarez, B.]] | [[Category: Garcia-Alvarez, B.]] | ||
[[Category: Ginsberg, M | [[Category: Ginsberg, M H.]] | ||
[[Category: Liddington, R | [[Category: Liddington, R C.]] | ||
[[Category: Pereda, J | [[Category: Pereda, J M.de.]] | ||
[[Category: Ulmer, T | [[Category: Ulmer, T S.]] | ||
[[Category: cytoskeleton]] | [[Category: cytoskeleton]] | ||
[[Category: ferm domain]] | [[Category: ferm domain]] | ||
| Line 26: | Line 26: | ||
[[Category: integrin binding]] | [[Category: integrin binding]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:34 2008'' | ||
Revision as of 14:55, 21 February 2008
|
Crystal structure of a FERM domain of Talin
OverviewOverview
The binding of cytoplasmic proteins, such as talin, to the cytoplasmic domains of integrin adhesion receptors mediates bidirectional signal transduction. Here we report the crystal structure of the principal integrin binding and activating fragment of talin, alone and in complex with fragments of the beta 3 integrin tail. The FERM (four point one, ezrin, radixin, and moesin) domain of talin engages integrins via a novel variant of the canonical phosphotyrosine binding (PTB) domain-NPxY ligand interaction that may be a prototype for FERM domain recognition of transmembrane receptors. In combination with NMR and mutational analysis, our studies reveal the critical interacting elements of both talin and the integrin beta 3 tail, providing structural paradigms for integrin linkage to the cell interior.
About this StructureAbout this Structure
1MIX is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
ReferenceReference
Structural determinants of integrin recognition by talin., Garcia-Alvarez B, de Pereda JM, Calderwood DA, Ulmer TS, Critchley D, Campbell ID, Ginsberg MH, Liddington RC, Mol Cell. 2003 Jan;11(1):49-58. PMID:12535520
Page seeded by OCA on Thu Feb 21 13:55:34 2008