1m6p: Difference between revisions

Revision as of 14:52, 21 February 2008

EXTRACYTOPLASMIC DOMAIN OF BOVINE CATION-DEPENDENT MANNOSE 6-PHOSPHATE RECEPTOR

OverviewOverview

Targeting of newly synthesized lysosomal hydrolases to the lysosome is mediated by the cation-dependent mannose 6-phosphate receptor (CD-MPR) and the insulin-like growth factor II/cation-independent mannose 6-phosphate receptor (IGF-II/CI-MPR). The two receptors, which share sequence similarities, constitute the P-type family of animal lectins. We now report the three-dimensional structure of a glycosylation-deficient, yet fully functional form of the extracytoplasmic domain of the bovine CD-MPR (residues 3-154) complexed with mannose 6-phosphate at 1.8 A resolution. The extracytoplasmic domain of the CD-MPR crystallizes as a dimer, and each monomer folds into a nine-stranded flattened beta barrel, which bears a striking resemblance to avidin. The distance of 40 A between the two ligand-binding sites of the dimer provides a structural basis for the observed differences in binding affinity exhibited by the CD-MPR toward various lysosomal enzymes.

About this StructureAbout this Structure

1M6P is a Single protein structure of sequence from Bos taurus with and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Molecular basis of lysosomal enzyme recognition: three-dimensional structure of the cation-dependent mannose 6-phosphate receptor., Roberts DL, Weix DJ, Dahms NM, Kim JJ, Cell. 1998 May 15;93(4):639-48. PMID:9604938

Page seeded by OCA on Thu Feb 21 13:52:08 2008

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OCA

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-[[Image:1m6p.jpg|left|200px]]<br /><applet load="1m6p" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1m6p.jpg|left|200px]]<br /><applet load="1m6p" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1m6p, resolution 1.8&Aring;" />
 '''EXTRACYTOPLASMIC DOMAIN OF BOVINE CATION-DEPENDENT MANNOSE 6-PHOSPHATE RECEPTOR'''<br />
 ==Overview==
-Targeting of newly synthesized lysosomal hydrolases to the lysosome is, mediated by the cation-dependent mannose 6-phosphate receptor (CD-MPR) and, the insulin-like growth factor II/cation-independent mannose 6-phosphate, receptor (IGF-II/CI-MPR). The two receptors, which share sequence, similarities, constitute the P-type family of animal lectins. We now, report the three-dimensional structure of a glycosylation-deficient, yet, fully functional form of the extracytoplasmic domain of the bovine CD-MPR, (residues 3-154) complexed with mannose 6-phosphate at 1.8 A resolution., The extracytoplasmic domain of the CD-MPR crystallizes as a dimer, and, each monomer folds into a nine-stranded flattened beta barrel, which bears, a striking resemblance to avidin. The distance of 40 A between the two, ligand-binding sites of the dimer provides a structural basis for the, observed differences in binding affinity exhibited by the CD-MPR toward, various lysosomal enzymes.
+Targeting of newly synthesized lysosomal hydrolases to the lysosome is mediated by the cation-dependent mannose 6-phosphate receptor (CD-MPR) and the insulin-like growth factor II/cation-independent mannose 6-phosphate receptor (IGF-II/CI-MPR). The two receptors, which share sequence similarities, constitute the P-type family of animal lectins. We now report the three-dimensional structure of a glycosylation-deficient, yet fully functional form of the extracytoplasmic domain of the bovine CD-MPR (residues 3-154) complexed with mannose 6-phosphate at 1.8 A resolution. The extracytoplasmic domain of the CD-MPR crystallizes as a dimer, and each monomer folds into a nine-stranded flattened beta barrel, which bears a striking resemblance to avidin. The distance of 40 A between the two ligand-binding sites of the dimer provides a structural basis for the observed differences in binding affinity exhibited by the CD-MPR toward various lysosomal enzymes.
 ==About this Structure==
-M6P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with MN and M6P as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M6P OCA].
+M6P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=M6P:'>M6P</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M6P OCA].
 ==Reference==
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 [[Category: Bos taurus]]
 [[Category: Single protein]]
-[[Category: Dahms, N.M.]]
+[[Category: Dahms, N M.]]
-[[Category: Kim, J.J.P.]]
+[[Category: Kim, J J.P.]]
-[[Category: Roberts, D.L.]]
+[[Category: Roberts, D L.]]
-[[Category: Weix, D.J.]]
+[[Category: Weix, D J.]]
 [[Category: M6P]]
 [[Category: MN]]
@@ Line 24: / Line 24: @@
 [[Category: transport]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:11:18 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:52:08 2008''