1m5p: Difference between revisions
New page: left|200px<br /> <applet load="1m5p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m5p, resolution 2.60Å" /> '''Transition State St... |
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[[Image:1m5p.gif|left|200px]]<br /> | [[Image:1m5p.gif|left|200px]]<br /><applet load="1m5p" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1m5p" size=" | |||
caption="1m5p, resolution 2.60Å" /> | caption="1m5p, resolution 2.60Å" /> | ||
'''Transition State Stabilization by a Catalytic RNA'''<br /> | '''Transition State Stabilization by a Catalytic RNA'''<br /> | ||
==Overview== | ==Overview== | ||
The hairpin ribozyme catalyzes sequence-specific cleavage of RNA through | The hairpin ribozyme catalyzes sequence-specific cleavage of RNA through transesterification of the scissile phosphate. Vanadate has previously been used as a transition state mimic of protein enzymes that catalyze the same reaction. Comparison of the 2.2 angstrom resolution structure of a vanadate-hairpin ribozyme complex with structures of precursor and product complexes reveals a rigid active site that makes more hydrogen bonds to the transition state than to the precursor or product. Because of the paucity of RNA functional groups capable of general acid-base or electrostatic catalysis, transition state stabilization is likely to be an important catalytic strategy for ribozymes. | ||
==About this Structure== | ==About this Structure== | ||
1M5P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1M5P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5P OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Amare, A | [[Category: Amare, A R.Ferre-D.]] | ||
[[Category: Massey, A.]] | [[Category: Massey, A.]] | ||
[[Category: Rupert, P | [[Category: Rupert, P B.]] | ||
[[Category: Sigurdsson, S | [[Category: Sigurdsson, S T.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
[[Category: catalytic rna]] | [[Category: catalytic rna]] | ||
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[[Category: u1a rna binding protein]] | [[Category: u1a rna binding protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:51:43 2008'' | ||
