1m5o: Difference between revisions
New page: left|200px<br /> <applet load="1m5o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m5o, resolution 2.20Å" /> '''Transition State St... |
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[[Image:1m5o.gif|left|200px]]<br /> | [[Image:1m5o.gif|left|200px]]<br /><applet load="1m5o" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1m5o, resolution 2.20Å" /> | caption="1m5o, resolution 2.20Å" /> | ||
'''Transition State Stabilization by a Catalytic RNA'''<br /> | '''Transition State Stabilization by a Catalytic RNA'''<br /> | ||
==Overview== | ==Overview== | ||
The hairpin ribozyme catalyzes sequence-specific cleavage of RNA through | The hairpin ribozyme catalyzes sequence-specific cleavage of RNA through transesterification of the scissile phosphate. Vanadate has previously been used as a transition state mimic of protein enzymes that catalyze the same reaction. Comparison of the 2.2 angstrom resolution structure of a vanadate-hairpin ribozyme complex with structures of precursor and product complexes reveals a rigid active site that makes more hydrogen bonds to the transition state than to the precursor or product. Because of the paucity of RNA functional groups capable of general acid-base or electrostatic catalysis, transition state stabilization is likely to be an important catalytic strategy for ribozymes. | ||
==About this Structure== | ==About this Structure== | ||
1M5O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1M5O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5O OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Amare, A | [[Category: Amare, A R.Ferre-D.]] | ||
[[Category: Massey, A | [[Category: Massey, A P.]] | ||
[[Category: Rupert, P | [[Category: Rupert, P B.]] | ||
[[Category: Sigurdsson, S | [[Category: Sigurdsson, S T.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
[[Category: catalytic rna]] | [[Category: catalytic rna]] | ||
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[[Category: vandate]] | [[Category: vandate]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:51:48 2008'' | ||
Revision as of 14:51, 21 February 2008
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Transition State Stabilization by a Catalytic RNA
OverviewOverview
The hairpin ribozyme catalyzes sequence-specific cleavage of RNA through transesterification of the scissile phosphate. Vanadate has previously been used as a transition state mimic of protein enzymes that catalyze the same reaction. Comparison of the 2.2 angstrom resolution structure of a vanadate-hairpin ribozyme complex with structures of precursor and product complexes reveals a rigid active site that makes more hydrogen bonds to the transition state than to the precursor or product. Because of the paucity of RNA functional groups capable of general acid-base or electrostatic catalysis, transition state stabilization is likely to be an important catalytic strategy for ribozymes.
About this StructureAbout this Structure
1M5O is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Transition state stabilization by a catalytic RNA., Rupert PB, Massey AP, Sigurdsson ST, Ferre-D'Amare AR, Science. 2002 Nov 15;298(5597):1421-4. Epub 2002 Oct 10. PMID:12376595
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