1m4m: Difference between revisions
New page: left|200px<br /><applet load="1m4m" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m4m, resolution 2.80Å" /> '''Mouse Survivin'''<br... |
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[[Image:1m4m.jpg|left|200px]]<br /><applet load="1m4m" size=" | [[Image:1m4m.jpg|left|200px]]<br /><applet load="1m4m" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1m4m, resolution 2.80Å" /> | caption="1m4m, resolution 2.80Å" /> | ||
'''Mouse Survivin'''<br /> | '''Mouse Survivin'''<br /> | ||
==Overview== | ==Overview== | ||
The coupling of apoptosis (programmed cell death) to the cell division | The coupling of apoptosis (programmed cell death) to the cell division cycle is essential for homeostasis and genomic integrity. Here, we report the crystal structure of survivin, an inhibitor of apoptosis, which has been implicated in both control of cell death and regulation of cell division. In addition to a conserved N-terminal Zn finger baculovirus IAP repeat, survivin forms a dimer through a symmetric interaction with an intermolecularly bound Zn atom located along the molecular dyad axis. The interaction of the dimer-related C-terminal alpha helices forms an extended surface of approximately 70 A in length. Mutagenesis analysis revealed that survivin dimerization and an extended negatively charged surface surrounding Asp-71 are required to counteract apoptosis and preserve ploidy. These findings may provide a structural basis for a dual role of survivin in inhibition of apoptosis and regulation of cell division. | ||
==About this Structure== | ==About this Structure== | ||
1M4M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1M4M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M4M OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Altieri, D | [[Category: Altieri, D C.]] | ||
[[Category: Chen, J.]] | [[Category: Chen, J.]] | ||
[[Category: Jakob, C.]] | [[Category: Jakob, C.]] | ||
[[Category: Li, F.]] | [[Category: Li, F.]] | ||
[[Category: Matayoshi, E | [[Category: Matayoshi, E D.]] | ||
[[Category: Muchmore, S | [[Category: Muchmore, S W.]] | ||
[[Category: Ng, S | [[Category: Ng, S C.]] | ||
[[Category: Wu, W.]] | [[Category: Wu, W.]] | ||
[[Category: Zakula, D.]] | [[Category: Zakula, D.]] | ||
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[[Category: zn finger baculovirus iap repeat]] | [[Category: zn finger baculovirus iap repeat]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:51:23 2008'' | ||
Revision as of 14:51, 21 February 2008
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Mouse Survivin
OverviewOverview
The coupling of apoptosis (programmed cell death) to the cell division cycle is essential for homeostasis and genomic integrity. Here, we report the crystal structure of survivin, an inhibitor of apoptosis, which has been implicated in both control of cell death and regulation of cell division. In addition to a conserved N-terminal Zn finger baculovirus IAP repeat, survivin forms a dimer through a symmetric interaction with an intermolecularly bound Zn atom located along the molecular dyad axis. The interaction of the dimer-related C-terminal alpha helices forms an extended surface of approximately 70 A in length. Mutagenesis analysis revealed that survivin dimerization and an extended negatively charged surface surrounding Asp-71 are required to counteract apoptosis and preserve ploidy. These findings may provide a structural basis for a dual role of survivin in inhibition of apoptosis and regulation of cell division.
About this StructureAbout this Structure
1M4M is a Single protein structure of sequence from Mus musculus with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure and mutagenic analysis of the inhibitor-of-apoptosis protein survivin., Muchmore SW, Chen J, Jakob C, Zakula D, Matayoshi ED, Wu W, Zhang H, Li F, Ng SC, Altieri DC, Mol Cell. 2000 Jul;6(1):173-82. PMID:10949038
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